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Human Synoviocytes (HSn)
Description
Catalogue Number
Human Synoviocytes – cryopreserved
19-SYN
Human Synoviocytes – proliferating in Tflascs
19-SYN-T
Presentation
TCS human synoviocytes are fibroblast-like cells isolated from the synovial tissues of patients with rheumatoid
arthritis undergoing joint replacement procedures. These cells are known to contribute to joint destruction by
release of inflammatory cytokines and matrix metalloproteinases.
Cryopreserved: Each vial of cryopreserved human synoviocytes contains a minimum of 500,000 cells, guaranteed
to be
³70% viable after thawing. Cells are frozen at the end of the primary culture in a medium containingcryoprotectant.
Proliferating: Proliferating HSns are supplied as sub-confluent monolayers in their second passage. Flasks are
completely filled with medium to prevent mechanical damage to cells at the air/liquid interface during shipping.
Recommended Media
SynovioMax or Human Synoviocyte Growth Supplement 19-SYNMAX)
Recommended Seeding Density
2,500-5000 viable cells per cm
2Proliferation capacity
Normal human cells have a limited life span
in vitro. When cells are cultured using our recommended reagents andprocedures, GENTAUR guarantee cryopreserved human synoviocytes for
³10 population doublings, andproliferating cells for 7 population doublings.
Storage and maintenance of cells
Cryopreserved and proliferating cells must be dealt with according to the following guidelines
immediately on theirreceipt:
Cryopreserved human synoviocytes will arrive frozen on dry ice, and if not seeded immediately must be
transferred to the vapour phase of a liquid nitrogen storage facility.
N.B. Continued storage on dry ice or at –70oC isNOT appropriate.
Proliferating cultures of human synoviocytes cells must be transferred to 37
°C and dealt with according to theguidelines sent with this data sheet
Batch Specific Information
For all batch specific information, including donor information, characterisation of cells and cell viability please refer
to the accompanying certificate of analysis
For research use only, not for diagnostic or therapeutic use.
