GENTAUR EUROPE  +32 1658 9045 +32 1650 9045BELGIUM1 tel +32 2 732 5688 fax +32 2 732 4414 [email protected] Av. de l' Armée 68 B-1040 Brussels France tel 01 43 25 01 50 fax 01 43 25 01 60 9, rue Lagrange 75005 Paris Italy tel 02 36 00 65 93 fax +32 16 50 90 45 20135 Milano Germany tel +32 16 58 90 45 fax +32 16 50 90 45 Forckenbeckstraße 6 D-52074 Aachen Japan tel +81 78 386 0860 fax +81 78 306 0296 Minaatojimaminami-manchi Chuo-ku, Kobe 065-0047  |
|
Normal Human Chondrocytes 19-CHON Normal Human Chondrocytes are used forexperimental applications in joint research, physiology, cellular function and differentiation. Cryopreserved 19-CHON are shipped in second passage. Proliferating 19-CHON are shipped in third passage in T-flasks. Chondrocytes are specialized cells which produce and maintain the extracellular matrix of cartilage and are the only cellular component of cartilage. Although cartilage is not confined to articulating joints, articular cartilage, especially from weight bearing joints, is an area of intense research because arthritis, joint injuries and joint abnormalities cause wide spread disability. GENTAUR Chondrocyte Cell are convenient and easy to use, allowing the researcher to focus on results. Chondrocytes are differentiated cells when isolated. They lose their differentiated phenotype after serial expansion in monolayer culture. By the second or third passage the cells are almost completely dedifferentiated. Chondrocytes can be expanded in culture and then induced to re-differentiate. In addition ChondroMaxTM is designed to generate 19-CHON cultures for experimental applications including studies on the formation, breakdown, and regeneration of hyaline cartilage, Osteoarthritis research and the proliferation and differentiation of chondrocytes. GENTAUR cells, medium and reagents are quality tested together and guaranteed to give optimum performance as a complete "cell system". Cell System Components · One Normal Human Chondrocyte Cell Product(Cryopreserved or Proliferating) · One Normal Human Chondrocyte Cell GrowthMedium supplement ChondroMax 25 ml
Characterization of Cells Cells are tested for the presence of Collagen Type II at primary and/or after re-differentiation. Cells are tested for sulfated proteoglycans with Alcian Blue stain after re-differentiation. Please note, the differentiation system does not attempt to redifferentiate the cells to hypertrophic state. The cells are re-differentiated to produce Collagen Type II but do not necessarily produce Collagen Type X upon re-differentiation. Performance Recommended Seeding Density 10,000 cells/cm² Typical time from subculture to confluent monolayer 4 - 9 days Cumulative population doublings guaranteed using GENTAUR system 15 Quality Control All cells are performance assayed and test negative for HIV-1, mycoplasma, Hepatitis-B, Hepatitis-C, bacteria, yeast and fungi. Cell viability, morphology and proliferative capacity are measured after recovery from cryopreservation. GENTAUR media are optimized to grow specific types of normal human cells. Each lot of medium is tested for the support of cell viability and proliferative capacity. Certificates of Analysis (CoA) for each cell strain are shipped with each order. CoA for all other products are available upon request. |
