Sera and Media

Sera and Media

	SERA		SEPERATING SOLUTIONS & LECTINS
	CELL CULTURE MEDIA		ANTIBIOTICS
	COMPLETE CULTURE MEDIA		BALANCED SALTS & BUFFERS
	CELL ATTACHMENT FACTORS		CELL CULTURE REAGENTS
	TISSUE DISINTEGRATION ENZYMES		CELL CULTURE DIAGNOSTICS

SERA

Fetal Bovine Serum (FBS)

BIOCHROM AG, Germany offers FBS, originated from four different sources, Australia , USA , EU- and USDA – approved countries. FBS offered by BIOCHROM AG are tested for Endotoxin, Mycoplasma, Bacterial L-forms, Viral contamination, Viral antibodies against BVD-MD, BHV-1, PI-3.

BIOCHROM AG also offers pre-tested FBS lots suitable for Amniotic Fluid cells, Hybridoma cells and Embryonic Stem cells.

Ordering Information
Cat.No	Product	Units
S 0113	Fetal Bovine Serum (FBS) (origin: EU-approved countries)	100ml
S 0115	Fetal Bovine Serum (FBS) (origin: EU-approved countries)	500ml
S 0213	Fetal Bovine Serum (FBS) (origin: USDA-countries)	100ml
S 0215	Fetal Bovine Serum (FBS) (origin: USDA-countries)	500ml
S 0313	Fetal Bovine Serum (FBS) (origin: USA)	100ml
S 0315	Fetal Bovine Serum (FBS)(origin: USA)	500ml
S 0413	Fetal Bovine Serum (FBS) (origin: Australia)	100ml
S 0415	Fetal Bovine Serum (FBS) (origin: Australia)	500ml

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Cell Culture Media

BIOCHROM liquid and powdered cell culture media are standardized according to the original formulation as recommended by the 'Tissue CultureAssociation'.Any modifications from the original formulation are indicated on the label.

Defined cell culture media generally consist of four basic chemical groups: amino acids, carbohydrates, anorganic salts and vitamins. The following is the list of Cell culture media offered by BIOCHROM.

Ordering Information
Cat.No	Product	Units
F 0915	Alpha Medium with 2.0g/l NaHCO₃ with nucleosides	500ml
F 0925	Alpha Medium with 2.0g/l NaHCO₃ without nucleosides	500ml
F 0225	BME with Earle's Salts with 2.2g/l NaHCO₃	500ml
F 0235	BME with Earle's Salts (10X) without NaHCO₃	500ml
F 0245	BME with Hanks' Salts with 0.35g/l NaHCO₃	500ml
F 0565	CMRL 1066 with 2.2g/l NaHCO₃	500ml
F 0855	Coon's F-12 mod. with 2.5g/l NaHCO₃, without L-Glutamine	500ml
F 0405	DMEM with 3.7g/l NaHCO₃, without Glucose	500ml
F 0413	DMEM with 3.7g/l NaHCO₃, with 1.0g/l Glucose	100ml
F 0415	DMEM with 3.7g/l NaHCO₃, with 1.0g/l Glucose	500ml
FG 0415	DMEM with 3.7 g/l NaHCO₃, with 1.0g/l Glucose, with stable glutamine	500ml
F 0425	DMEM B723w.3.7g/l NaHCO₃, w.4.5g/l Gluc, w/oL-Glut.w/oNa-Pyr.w.20mM HEPES 3	500ml
F 0435`	DMEM with 3.7g/l NaHCO₃, with 4.5g/l Glucose w/oNa-Pyruvate.	500ml
FG 0435	DMEM with 3.7g/l NaHCO₃, with 4.5g/l Glucose, w.stable glutamine w/o Na-Pyrv	500ml
F 0445	DMEM with 3.7 g/l NaHCO₃, with 4.5 g/l Glucose with Na-Pyruvate.	500ml
FG 0445	DMEM with 3.7g/l NaHCO₃, with 4.5g/l Glucose w.stable glutamine w.Na-Pyrv	500ml
F 0455	DMEM (10X) with 4.5g/l Glucose, without NaHCO₃, w/oL-Glut., w/o Na-Pyrv	500ml
F 1445	VLE-DMEM (low endotoxn) w 4.5g/l gluc,w 3.7g/l NaHCO₃, wNa-Pyr	500ml
FG 1445	VLE-DMEM w 4.5g/l gluc, w 3.7g/l NaHCO₃, w Na-Pyr,w stab glut	500ml
F 0475	DMEM with 4.5g/l Glucose, with Na-Pyruvate, w/o Phenol red	500ml
T 041-01	DMEM Powder Medium with 1.0g/l Glucose	1litre
T 041-05	DMEM Powder Medium with 1.0g/l Glucose	5litres
T 041-10	DMEM Powder Medium with 1.0g/l Glucose	10litres
T 041-50	DMEM Powder Medium with 1.0g/l Glucose	50litres
T 043-01	DMEM Powder Medium with 4.5g/l Glucose	1litre
T 043-05	DMEM Powder Medium with 4.5g/l Glucose	5litres
T 043-10	DMEM Powder Medium with 4.5g/l Glucose	10litres
T 043-50	DMEM Powder Medium with 4.5g/l Glucose	50litres
F 4815	DMEM/Ham's F-12 (1:1) with 4.5g/l Glucose	500ml
FG 4815	DMEM/Ham's F-12 (1:1) with 4.5g/l Glucose with stable glutamine	500ml
T 481-01	DMEM/Ham's F-12 (1:1) Powder Medium with 4.5g/l Glucose	1litre
T 481-05	DMEM/Ham's F-12 (1:1) Powder Medium with 4.5g/l Glucose	5litres
T 481-10	DMEM/Ham's F-12 (1:1) Powder Medium with 4.5g/l Glucose	10litres
T 481-50	DMEM/Ham's F-12 (1:1) Powder Medium with 4.5g/l Glucose	50litres
F 0525	Glasgow-MEM with 2.A15675 g/l NaHCO₃	500ml
F 0555	Grace's Insect cell medium	500ml
F 0713	Ham's F-10 with 10mg/l phenol red, with 1.2g/l NaHCO₃	100ml
F 0715	Ham's F-10 with 10mg/l phenol red, with 1.2g/l NaHCO₃	500ml
FG 0713	Ham's F-10 with 10mg/l phenol red, with 1.2g/l NaHCO₃, with stable glutamine	100ml
FG 0715	Ham's F-10 with 10mg/l phenol red, with 1.2g/l NaHCO₃, with stable glutamine	500ml
F 0723	Ham's F-10 without phenol red, with 1.2g/l NaHCO₃, with 10mM HEPES	100ml
T 071-01	Ham's F-10 Powder Medium	1litre
T 071-05	Ham's F-10 Powder Medium	5litres
T 071-10	Ham's F-10 Powder Medium	10litres
T 071-50	Ham's F-10 Powder Medium	50litres
F 0815	Ham's F-12 with 10 mg/l phenol red, with 1.176g/l NaHCO₃	500ml
FG 0815	Ham's F-12 with 10 mg/l phenol red, w. 1.176g/l NaHCO₃, with stable glutamine	500ml
F 0465	Iscove's Medium, not compl., w.15mg/l phenol red, w. 3.024g/l NaHCO₃	500ml
FG 0465	Iscove's Medium, not compl.,w.15mg/l phenol red, w.3.024g/l NaHCO₃, w.Glut.B827	500ml
F 1315	L-15 Leibovitz Medium	500ml
F 1013	Mc-COY's 5 A mod. Medium with 2.2 g/l NaHCO₃	100ml
F 1015	Mc-COY's 5 A mod. Medium with 2.2 g/l NaHCO₃	500ml
F 0613	Medium 199 with Earle's Salts, with 2.2 g/l NaHCO₃	100ml
F 0615	Medium 199 with Earle's Salts, with 2.2 g/l NaHCO₃	500ml
FG 0615	Medium 199 with Earle's Salts, with 2.2 g/l NaHCO₃, with stable glutamine	500ml
F 0633	Medium 199 with Hanks' Salts, with 0.35 g/l NaHCO₃	100ml
F 0635	Medium 199 with Hanks' Salts, with 0.35 g/l NaHCO₃	500ml
F 0663	Medium 199 with Earle's Salts, with 20mM HEPES without NaHCO₃	100ml
F 0665	Medium 199 with Earle's Salts, with 20mM HEPES without NaHCO₃	500ml
T 061-01	Medium 199 - Powder Medium with Earle's Salts	1litre
T 061-05	Medium 199 - Powder Medium with Earle's Salts	5litres
T 061-10	Medium 199 - Powder Medium with Earle's Salts	10litres
T 061-50	Medium 199 - Powder Medium with Earle's Salts	50litres
F 0315	MEM with Earle's Salts with 0.85 g/l NaHCO₃	500ml
F 0325	MEM with Earle's Salts with 2.2 g/l NaHCO₃	500ml
FG 0325	MEM with Earle's Salts with 2.2 g/l NaHCO₃ with stable glutamine	500ml
F 0333	MEM with Earle's Salts (10X) without NaHCO₃	100ml
F 0335	MEM with Earle's Salts (10X) without NaHCO₃	500ml
F 0345	MEM with Hanks' Salts with 0.35 g/l NaHCO₃	500ml
F 0383	MEM with Earle's Salts w/o NaHCO₃, w/o phenol red	100ml
F 0385	MEM with Earle's Salts w/o NaHCO₃, w/o phenol red	500ml
F 4315	MEM with Earle's Salts with 20mM HEPES without NaHCO₃	500ml
T 437-01	MEM-Powder Medium with Earle's Salts with non-essential amino acids	1litre
T 437-05	MEM-Powder Medium with Earle's Salts with non-essential amino acids	5litres
T 437-10	MEM-Powder Medium with Earle's Salts with non-essential amino acids	10litres
T 437-50	MEM-Powder Medium with Earle's Salts with non-essential amino acids	50litres
F 1213	RPMI 1640 with 2.0g/l NaHCO₃, without L-Glutamine	100ml
F 1215	RPMI 1640 w. 2.0g/l NaHCO₃, w/o L-Glutamine	500ml
FG 1213	RPMI 1640 with 2.0g/l NaHCO₃, with stable glutamine	100ml
FG 1215	RPMI 1640 with 2.0g/l NaHCO₃, w stable glut. (also available as “low endotoxin”)	500ml
F 1223	RPMI 1640 (10x) without NaHCO₃	100ml
F 1225	RPMI 1640 (10x) without NaHCO₃	500ml
F 1233	RPMI 1640 with 20mM HEPES, without NaHCO₃	100ml
F 1235	RPMI 1640 with 20mM HEPES, without NaHCO₃	500ml
FG 1233	RPMI 1640 without NaHCO₃, with 20mM HEPES, with stable glutamine	100ml
FG 1235	RPMI 1640 without NaHCO₃, with 20mM HEPES, with stable glutamine	500ml
F 1245	RPMI 1640 with 2.0g/l NaHCO₃, without Methionine	500ml
F 1255	RPMI 1640 with 2.0g/l NaHCO₃, with 40mg/l folic acid	500ml
FG 1255	RPMI 1640 with 2.0g/l NaHCO₃, with 40mg/l folic acid, with stable glutamine	500ml
F 1275	RPMI 1640 with 2.0g/l NaHCO₃ without phenol red	500ml
FG 1383	RPMI 1640 w 2.0g/l NaHCO₃, 25mM HEPES, 5.5g/l NaCl, 5mg/l phenol red, stable glut.	100ml
FG 1385	RPMI 1640 w 2.0g/l NaHCO₃, 25mM HEPES, 5.5g/l NaCl, 5mg/l phenol red, stable glut.	500ml
F 1295	RPMI 1640 with 2.0g/l NaHCO₃, with 5mg/l phenol red	500ml
F 1415	VLE-RPMI 1640 (very low endotoxin), Endotoxin<0,01 IU/ml	500ml
FG 1415	VLE-RPMI 1640 (very low endotoxin) Endotoxin<0,01 IU/ml with stable glut.	500ml
T 121-01	RPMI 1640 - Powder Medium	1litre
T 121-05	RPMI 1640 - Powder Medium	5litres
T 121-10	RPMI 1640 - Powder Medium	10litres
T 121-50	RPMI 1640 - Powder Medium	50litres
F 0545	Insect cell medium TC 100	500ml
F 1115	Williams' Medium E with 2.2g/l NaHCO₃, without L-Glutamine	500ml
F 1125	Williams' Medium E with 2.2g/l NaHCO₃, without L-Glutamine, without phenol red	500ml

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Complete Culture Media

The Complete cell culture media are serum and protein free cell culture media which support the growth of different cells / cell lines. There are specific complete culture media are available to support the growth of cells / cell lines like, human amniotic fluid cells, FRTL 5, human and porcine hepatocytes, hybridomas, lymphocytes, human keratinocytes, CHO cells, VERO cells, sebocytes, neuronal cells, neuroblastoma x glioma hybrid cells, cytotoxicT-cells, chondrocytes, human endothelial cells etc.

Ordering Information
Cat.No	Product	Units
F 5043	Amniomed	100ml
F 5143	Amniomed with 25 mM HEPES	100ml
F 5013	Chromosome medium A without Phytohemagglutinin (PHA) L	100ml
F 5023	Chromosome A110medium B with Phytohemagglutinin (PHA) E	100ml
F 2235	Chondromed complete	500ml
F 2245	endomed complete	500ml
L 6211	Colchicin, 10 ug/ml in PBS, without Ca, Mg	25ml
L 6221	Colcemide, 10 ug/ml in PBS without Ca, Mg, in plastic bottles	25ml
L 6231	Colcemide, 10 µg/ml in HBSS	25ml.
F 8085	octomed serum free CHO-Medium with 2.2 g/l NaHCO₃, without L-Glutamine	500ml
F 2225	fibromed complete	500ml
F 2255	heparmed Liver cell Medium	500ml
F 5515	Hybridoma Medium without HAT, with L-Glutamine, with 10% FBS	500ml
F 8035	HybridoMed DIF1000 serum free medium, for 10%CO2 conc., in plastic bottles	500ml
F 8055	HybridoMed DIF1000 serum free+A201medium, for 5%CO2 conc.,in plastic bottl.	500ml
F 8105	MCDB 153 Basal Medium without L-Glutamine	500ml
F 8115	MCDB 153 Basal Medium with L-Glutamine, with 1.176 g/l NaHCO₃	500ml
F 2265	Osteomed complete	500ml
F 8045	PFEK-1, protein free Medium for VERO-cells	500ml
F 8205	sebomed Basal Medium	500ml
F 8215	sebomed Complete Medium	500ml
F 8075	Start V Medium	500ml
F 8023	TNB 100 Medium in plastic bottles	100ml
F 8820	TNB 100, Lipid-Protein-Complex (necessary for 100 ml TNB 100)	2.5ml
F 0483	HAT-Supplement	100ml

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Cell Attachment Factors

Growth and differentiation of anchorage dependent cells are strongly influenced by the glass or plastic culture flasks offered as substrate. Cell growth rates can often be improved by surface coating with attachment factors such as fibronectin, collagen, gelatine or polylysine. With a collagen coating, survival time of hepatocytes cab be extended from normally one week to four weeks.

Ordering Information
Cat.No	Product	Units
L 7117	Fibronectin from human plasma	1mg
L 7127	Fibronectin from bovine plasma	1mg
L 7220	Collagen A, 1mg/ml	1 x (6 x 5ml)
L 7213	Collagen G	100ml
L 7230	Gelatine 10mg/ml	1 x (6 x 5ml)
L 7240	Polylysin MG>30.000; 0.1mg/ml	1 x (6 x 5ml)

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Tissue Disintegration Enzymes

Cells of animal tissue are attached to each other by a complex matrix of proteins, lipids, glycolipids and muco-polysaccharides. To isolate individual cells or to stat primary cultures, the delicate matrix must be partially broken down without destroying the cell or its surface.

Collagenase are the enzymes of choice for this application, if dissolved in balanced salts.

Ordering Information
Cat.No	Product	Units
C 1-28	Collagenase type I, CLS	100mg
C 1-22	Collagenase type I, CLS	1g
C 2-28	Collagenase type II, CLS II	100mg
C 2-22	Collagenase type II, CLS II	1g
C 3-28	Collagenase type III, CLS III	100mg
C 3-22	Collagenase type III, CLS III	1g
C 4-28	Collagenase type IV, CLS IV	100mg
C 4-22	Collagenase type IV, CLS IV	1g
L 2113	EDTA (Versen) 1% in PBS without Ca²⁺, Mg²⁺	100ml
L 2123	Trypsin (1:250) 0.25% in PBS without Ca²⁺, Mg²⁺	100ml
L 2133	Trypsin (1:250) 2.5% in PBS without Ca²⁺, Mg²⁺	100ml
L 2153	Trypsin/EDTA Solution(10X)0.5%/0.2% in (10x) PBS,w/o Ca²⁺, Mg²⁺, in pl.B911 bott.	100ml
L 2163	Trypsin/EDTA solution 0.25%/0.02% in PBS without Ca²⁺, Mg²⁺	100ml
L 2103	Trypsin powder substance	100g
L 2223	Papain Solution (6.25mg/l)	100ml

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Seperating Solutions & Lectins

Collagenase are the enzymes of choice for this application, if dissolved in balanced salts.

Ordering Information
Cat.No	Product	Units
L 6113	Biocoll-Separating Solution, density 1.077g/ml isoton	100ml
L 6115	Biocoll-Separating Solution, density 1.077g/ml isoton	500ml
L 6125	Biocoll-Separating Solution, density 1.09g/ml isoton	500ml
L 6155	Biocoll-Separating Solution, density 1.10g/ml isoton	500ml
L 6133	Percoll Separating Solution, density 1.077g/ml isoton	100ml
L 6135	Percoll Separating Solution, density 1.077g/ml isoton	500ml
L 6143	Percoll Separating Solution, density 1.124g/ml isoton	100ml
L 6145	Percoll Separating Solution, density 1.124g/ml isoton	500ml
L 6163	Percoll Separating Solution 40 %, density 1.055g/ml at +20°C	100ml
L 6173	Percoll Separating Solution 80 %, density 1.02g/ml at +20°C	100ml
M 5030	Phytohemagglutinine PHA L	1x(6x5ml)
M 5040	Phytohemagglutinine PHA E	1x(6x5ml)
M 5050	Concanavalin A (Con A)	1x(6x5ml)
M 5060	Pokeweed mitogen (PWM)	1x(6x5ml)

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Antibiotics

Cell culture media provide an ideal environment also for rods such as bacteria and fungi. Basically, cell cultures can be protected against contamination by strict adherence to sterile technique; but only the discovery of antibiotics made advanced cell culture

possible. However, as antibiotics have a toxic potental, their dosage must closely be monitored.

Ordering Information
Cat.No	Product	Units
A 2610	Amphotericin B, 250 µg/ml, lyophilized	1 x (6 x 5ml)
A 2612	Amphotericin B, 250 µg/ml	50ml
A 2812	Partricin	50ml
A 2710	Gentamycin 10 mg/ml, lyophilized	1 x (6 x 5ml)
A 2712	Gentamycin 10 mg/ml	50ml
A 271-23	Gentamycin-Sulfate	2.5g
A 271-25	Gentamycin-Sulfate	10g
A 271-26	Gentamycin-Sulfate	25g
A 2512	Kanamycin 5 mg/ml	50ml
A 231-40	Polymyxin B-Sulfate, approx. 6.000 - 8.000 U/mg	5 Mio IE
A 2412	Neomycin/Bacitracin 50 U/ml	50ml
A 2210	Penicillin/Streptomycin 10.000 U/10.000 µg/ml, lyophilized	1 x (6 x 5ml)
A 2212	Penicillin/Streptomycin 10.000 U/10.000 µg/ml	50ml
A 2213	Penicillin/Streptomycin 10.000 U/10.000 µg/ml	100ml
A 321-42	Penicillin	25 Mio IE
A 321-44	Penicillin	100 Mio IE
A 331-26	Streptomycin –Sulfate	25g
A 331-27	Streptomycin –Sulfate	100g

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Balanced Salts & Buffers

Ordering Information
Cat.No	Product	Units
L 6015	Alsever Solution pH 6.1	500ml
L 1913	Earle's Saline Solution (EBSS) with 2.2g/l NaHCO₃	100ml
L 1915	Earle's Saline Solution (EBSS) with 2.2g/l NaHCO₃	500ml
L 1925	Earle's Saline Solution (EBSS) (10X) without NaHCO₃	500ml
L 2013	Hanks' Saline Solution (HBSS) with 0.35g/l NaHCO₃	100ml
L 2015	Hanks' Saline Solution (HBSS) with 0.35g/l NaHCO₃	500ml
L 2025	Hanks' Saline Solution (HBSS) (10X) without NaHCO₃	500ml
L 2035	Hanks' Saline Solution (HBSS) without phenol red, with 0.35g/l NaHCO₃	500ml
L 2045	Hanks' Saline Solution (HBSS) w/o Ca²⁺, Mg²⁺,w/o phenol red,w.0.35g/l NaHCO₃	500ml
L 2053	Hanks' Saline Solution (HBSS) w/o Ca²⁺, Mg²⁺,w. phenol red, w.0.35g/l NaHCO₃	100ml
L 2055	Hanks' Saline Solution (HBSS) w/o Ca²⁺, Mg²⁺, w. phenol red,w.0.35g/l NaHCO₃	500ml
L 201-01	Hanks' Salts powder substance without NaHCO₃	1litre
L 201-05	Hanks' Salts powder substance without NaHCO₃	5litres
L 201-10	Hanks' Salts powder substance without NaHCO₃	10litres
L 201-50	Hanks' Salts powder substance without NaHCO₃	50litres
L 1613	HEPES buffer solution (1M) (50X)	100ml
L 1603	HEPES powder substance	100g
L 1713	Sodium bicarbonate (7.5%) NaHCO₃	100ml
L 1703	Sodium bicarbonate NaHCO₃ powder substance	100g
L 1815	PBS Solution with Ca²⁺, Mg²⁺	500ml
L 1823	PBS Solution without Ca²⁺, Mg²⁺	100ml
L 1825	PBS Solution without Ca²⁺, Mg²⁺	500ml
L 1835	PBS Solution (10X) without Ca²⁺, Mg²⁺	500ml
L 182-01	PBS-powder substance without Ca²⁺, Mg²⁺	1litre
L 182-05	PBS-powder substance without Ca²⁺, Mg²⁺	5litres
L 182-10	PBS-powder substance without Ca²⁺, Mg²⁺	10litres
L 182-50	PBS-powder substance without Ca²⁺, Mg²⁺	50litres
L 0015	Ultra Pure Water in glass bottle, sterile	500ml
L 0020	Ultra Pure Water, sterile, in Duran bottles suitable for HPLC	1litre
L 0030	Ultra Pure Water, not sterile	5litres
L 0040	Ultra Pure Water, not sterile	10litres

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Cell Culture Reagents

Ordering Information
Cat.No	Product	Units
L 6510	Heparin stabilizer free 5000U/ml lyophilized (for 5ml)	1 x (6 x 5ml)
L 0473	Sodium pyruvate 100mM	100ml
K 0293	Non-essential amino acids (NEA) (100X)	100ml
K 0363	MEM Amino Acids (50X)	100ml
K 0373	MEM Vitamins (100X)	100ml
K 0280	L-Glutamine (200mM)	1 x (24 x 2.5ml)
K 0281	L-Glutamine (200mM), lyophilized	1 x (6 x 5ml)
K 0282	L-Glutamine (200mM) in plastic bottles	50ml
K 0202	N-Acetyl-L-alanyl-L-glutamine, 200mM (stable glutamine)	50ml
L 3010	Biotect Protection Medium	24 x 2.5ml
L 6413	KCI solution (hypotonic) 0.075M	100ml

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Cell Culture Diagnostics

Ordering Information
Cat.No	Product	Units
F 0483	HAT-Supplement (50X)	100ml
F 0493	HT-Supplement (50X)	100ml
D 7001	Mycoplasma test - DNA-staining kit	1Kit
L 2203	Phenol red 0.5 %	100ml
L 6313	Neutral red (1:300 - 0.3%)	100ml
L 6323	Trypan blue 05+A2% in phys. Saline Solution	100ml

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send mail to [email protected] with questions or comments about this web site. Copyright © 2008 Gentaur Molecular Products Site powered by Acid Dragon (AC) Last modified: 05/19/16

send mail to [email protected] with questions or comments about this web site.
Copyright © 2008 Gentaur Molecular Products
Site powered by Acid Dragon (AC)
Last modified: 05/19/16