Anti-PRG3 Polyclonal Antibody

 Catalog Number:         24345                                                     Price:  299 Euro/ 100ug

 Lot Number:                 R5601 

Quantity:                      100 mg in 100 ml (1 mg/ml) in PBS containing 0.02% sodium azide.

Description:                 The p53 tumor suppressor protein induces cell cycle arrest or apoptosis in response to cellular stresses. PRG3 (p53-responsive gene 3), is induced specifically under p53-dependent apoptotic conditions in human colon cancer cells.  PRG3 has significant homology to bacterial oxidoreductases and the apoptosis-inducing factor, AIF.  Unlike AIF, PRG3 localizes in the cytoplasm and its ectopic expression induces apoptosis. The PRG3 gene is thus a novel p53 target gene in a p53-dependent apoptosis pathway.

Host Species:               Rabbit

 Specificity:                   Reacts with residues 170-185 [VTLIHSQVALADKELL] of the 41 kDa human PRG3 protein.   This sequence is 87% identical in mouse.

Applications:                Western blot – Use at 1:500 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Format:                        Affinity purified IgG

Storage:                       Maintain at +2-8°C for 3 months or at -20°C for longer periods.  Stable for 1 year. Avoid repeated freeze-thaw cycles.

Reference:                   Ohiro Y. et al. FEBS Lett. 2002 Jul 31;524(1-3):163-71.