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Troponin I EIA Regulatory Status: For In-Vitro Diagnostic Use Discipline: Hypertension / Cardiac Evaluation Sample Type: Serum Size: 96 Wells Range: 1.5 - 100 ng/ml Sensitivity: 1.0 ng/ml Incubation Time: 1 hr and 50 min
Cardiac Troponin I is a protein found incardiac muscle, with a molecular weight of22.500 daltons. Together with Troponin T and C, it forms a structural complex which – aftercardiac damage – is released into blood circulation. Both free Troponin I and Troponin I in complex are measured. Troponin I is also found in skeletal muscles, but it differs in its amino acid composition from cardiac TroponinI. These two Troponins can be distinguished immunologically. • Troponin I is released into blood stream soon after onset of acute cardiac damage.Utilising sensitive immunological methods,a detectable level is reached after 3-6 hours after AMI. cTnI concentration in normal serum is below 0.1 ng/ml. Levels as high as 100 -300 ng/ml have been measured with some AMI patients.USE OF THE QUANTITATIVE ELISA KIT • The ELISA Troponin I test is used to examine human plasma or serum samples to detect elevated cTnI levels. • Ideal for use in laboratory environments of hospitals and health care centers. • The assay can be performed in just 75minutes. • No hook effect. • Detection time from 3-6 hours up to5-8 days from potential patient AMI. • Can be used with other diagnostic methods to detect myocardial damage. • No test-specific training is required. • Ready-to-use, stable liquid standards. • Clinical experiments show complete success in indicating cTnI positive samples, also incase of micro infarcts. |
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