GENTAUR EUROPE  +32 1658 9045 +32 1650 9045BELGIUM1 tel +32 2 732 5688 fax +32 2 732 4414 [email protected] Av. de l' Armée 68 B-1040 Brussels France tel 01 43 25 01 50 fax 01 43 25 01 60 9, rue Lagrange 75005 Paris Italy tel 02 36 00 65 93 fax +32 16 50 90 45 20135 Milano Germany tel +32 16 58 90 45 fax +32 16 50 90 45 Forckenbeckstraße 6 D-52074 Aachen Japan tel +81 78 386 0860 fax +81 78 306 0296 Minaatojimaminami-manchi Chuo-ku, Kobe 065-0047  |
 Also called: Apoptosis Inhibitor 4 or API4 Price: 655 Euro |
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Product Features | Significance and Uses |
Typical Standard Curves | Assay Procedure | Performance Specs | Cross Reactivities | Related Products |
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 FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
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Survivin poster presented at M.D. Anderson Cancer Center Tumor Markers
Conference, 2003... learn more>>
Survivin Range: 31.25-1,000 pg/mL |
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 Survivin, a new inhibitor of apoptosis (IAP) protein, is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, Survivin associates with the microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of Survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It indicates that Survivin may counteract a default induction of apoptosis in G2/M phase. The over expression of Survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis. |
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 1. Pipet Standards and Samples in duplicate into the wells and incubate. 2. Aspirate and wash the plate. 3. Pipet the yellow Antibody into the wells and incubate. 4. Aspirate and wash the plate. 5. Pipet the blue Conjugate into the wells and incubtate. 6. Aspirate and wash the plate. 7. Pipet Substrate into the wells and incubate. 8. Pipet Stop solution and read plate on a plate reader at 450nm. 9. Calculate sample concentrations from the Standard Curve.
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