Sulfonamides residues ELISA Kit

Brief
Sulfonamides residues (SAS) ELISA Test Kit

 Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Sulfamonomethoxine (SMM), Sulfadiazine (SD), Sulfamerazine (SM1), Sulfamethoxazole (SMZ) and Sulfathiazole (ST) in the tissue(chicken, pork), milk, honeyand egg. The coupling antigen is pre-coated on the micro-well stripes. The Sulfonamides residues in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against Sulfonamides. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample ha-s a negative correlation with the content of Sulfonamides residues in the sample. This value is compared to the standard curve and the content of the corresponding Sulfonamides residues is subsequently obtained.

Specifications
Sensitivity : 1ppb
Detection limit:
　　Meat(chicken,pork), liver(chicken,pork),honey, egg 1ppb
　　Serum, urine 4ppb
　　Milk 20ppb
Recovery rate
　　Meat(chicken,pork), liver(chicken,pork) 70 ±10%
　　Egg 65 ±10%
　　Milk ,honey,serum 70 ±10%
Cross-reaction rate:
Sulfadiazine(SD) 100%
Sulfamonomethoxine(SMM) 97.6%
Sulfamerazine(SM1) 12%
Sulfamethoxazole(SMZ) 63%
Sulfathiazole(ST) 195%
This test kit is used to detect 4 Sulfonamides based on cross-reaction rate, its detection limit
as follows: SD-1ppb SMM- 1ppb SMZ -2ppb ST -0.5ppb SM1-10ppb