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Recombinant Rat Interleukin 10 (rrIL-10)

 

Description

 

IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNFα. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on mouse cells, but mouse IL-10 is inactive on human cells. Recombinant rat IL-10 is an 18.7kDa protein of 160 amino acid residues

 

 

Specifications and Use

 

Source

 

l          Escherichia coli.

Molecular Mass

 

l          18kDa

 

Purity

 

l          Greater than 95% by SDS-PAGE analyses.

Biological Activity

l         The ED50 as determined by the dose-dependent inhibition of MC/9 proliferation is less than 10ng/ml.

 

Endotoxin Level

 

l          Less than 1EU/µg.

Formulation

 

l          Sterile filtered lyophilized (freeze-dried) powder.

 

Solubility

l          It is recommended to reconstitute the lyophilized rRaIL-10 in sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 1mg/ml of the cytokine.

 

Stability

 

l          The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20.

l          Reconstituted rat rrIL-10 should be stored in working aliquots at -20.

l          Avoid repeated freeze-thaw cycles.

 

Usage

l          FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.

 

 

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Last modified: 05/19/16