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recombinant Rat Interleukin 10
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Recombinant Rat Interleukin 10
(rrIL-10)
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Description
IL-10 is an immunosuppressive
cytokine produced by a variety of mammalian
cell types including macrophages, monocytes,
T cells, B cells and keratinocytes. IL-10
inhibits the expression of proinflammatory
cytokines such as IL-1 and TNFα. Like IL-4,
IL-10 enhances humoral immune responses and
attenuates cell-mediated immune reactions.
Human IL-10 active on mouse cells, but mouse
IL-10 is inactive on human cells.
Recombinant rat IL-10 is an 18.7kDa protein
of 160 amino acid residues
Specifications
and Use
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Source
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Escherichia coli. |
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Molecular Mass
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l
18kDa
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Purity
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l
Greater than 95% by SDS-PAGE analyses. |
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Biological Activity
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l
The ED50 as determined by the dose-dependent
inhibition of MC/9 proliferation is less
than 10ng/ml.
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Endotoxin Level
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Less than 1EU/µg. |
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Formulation
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l
Sterile filtered lyophilized (freeze-dried)
powder.
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Solubility
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l
It is recommended to
reconstitute the lyophilized rRaIL-10 in
sterile ddH2O containing at least
0.1% human serum albumin or bovine serum
albumin to prepare a stock solution of no
less than 1mg/ml of the cytokine.
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Stability
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l
The lyophilized protein is stable for a few
weeks at room temperature, but best stored
at -20℃.
l
Reconstituted rat rrIL-10 should be stored
in working aliquots at -20℃.
l
Avoid repeated freeze-thaw cycles.
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Usage |
l
FOR RESEARCH USE ONLY.
NOT FOR HUMAN USE. |
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Last modified:
05/19/16
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