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recombinant Mouse Interleukin 4
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Recombinant Mouse Interleukin 4 (rmIL-4)
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Description
IL-4 is a pleiotropic cytokine that
regulates diverse T and B cell responses
including cell proliferation, survival and
gene expression. Produced by mast cells, T
cells and bone marrow stromal cells, IL-4
regulates the differentiation of naive CD4+
T cells into helper Th2 cells, characterized
by their cytokine-secretion profile that
includes secretion of IL-4, IL-5, IL-6,
IL-10, and IL-13, which favor a humoral
immune response. Another dominant function
of IL-4 is the regulation of immunoglobulin
class switching to the IgG1 and IgE isotypes.
Excessive IL-4 production by Th2 cells has
been associated with elevated IgE production
and allergy. Recombinant mouse IL-4 is a
13.5kDa globular protein containing 120
amino acid residues.
Specifications
and Use
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Source
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E.coli |
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Molecular Mass
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13.5kDa.
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Purity
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l
> 95%, as determined by SDS-PAGE method. |
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Biological Activity
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l
The ED50 was determined by the
dose-dependent proliferation of mouse HT-2
cells is < 2.0ng/ml, corresponding to a
specific activity of > 5 x 105
units/mg.
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Endotoxin Level
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Less than 1EU/mg
of rmIL-4 as determined by LAL method. |
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Formulation
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Sterile filtered lyophilized (freeze-dried)
powder
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Solubility
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l
It is recommended to
reconstitute the lyophilized rmIL-4 in
sterile ddH2O containing at least
0.1% human serum albumin or bovine serum
albumin to prepare a stock solution of no
less than 5mg/ml
of the cytokine.
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Stability
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l
Lyophilized protein is stable for a few
weeks at room temperature, but best stored
at -20℃.
l
Reconstituted mouse IL-4 should be stored in
working aliquots at -20℃.
l
Avoid repeated freeze-thaw cycles.
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Usage |
l
FOR RESEARCH USE ONLY.
NOT FOR HUMAN USE.
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Last modified:
05/19/16
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