Description

IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNFα. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on mouse cells, but mouse IL-10 is inactive on human cells. Recombinant mouse IL-10 is an 18.7kDa protein of 161 amino acid residues.

Specifications and Use

Source

l          Escherichia coli.

Molecular Mass

l          18.7kDa

Purity

l          Greater than 95% by SDS-PAGE analyses.

Biological Activity

l          Mouse IL-10 is fully biologically active as measured by the dose-dependent co-stimulation (with IL-4) of the proliferation of mouse MC/9 cells. The ED50 was found to be < 2ng/ml, corresponding to a specific activity of > 5 x 10⁵ units/mg.

Endotoxin Level

l          Less than 1EU/µg.

Formulation

l        Sterile filtered lyophilized (freeze-dried) powder.

 

Solubility

l          It is recommended to reconstitute the lyophilized rmIL-10 in sterile ddH₂O containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 1mg/ml of the cytokine.

 

Stability

l          The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20℃.

l          Recombinant Mouse Interleukin rmIL-10 should be stored in working aliquots at -20℃.

l          Avoid repeated freeze-thaw cycles.

Usage

l          FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.