Description 

Flt3-Ligand is a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand has no activity on mast cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform.

Specifications and Use

Source

l          Escherichia coli.

 

Molecular Mass

l          16.4kDa

Purity

l          ³ 95% as determined by SDS-PAGE and HPLC analyses.

Biological Activity

l          rmFlt3-L is fully biologically active when compared to standards. Its specific activity is ³1×10⁵ units/mg.

 

Endotoxin Level

l          Less than 1EU/µg of rmFlt3-L as determined by LAL method.

Formulation

l          Sterile filtered lyophilized (freeze-dried) powder, with 50mM Tris,100mM NaCl，pH8.5

 

Solubility

l          It is recommended to reconstitute the lyophilized rmFlt3-L in sterile distilled H₂O to prepare a stock solution of no less than 100 mg/ml of the cytokine.  

Stability

l          The lyophilized rmFlt3-L powder is stable for a few weeks at room temperature, but best stored at -20℃.

l          Reconstituted rmFlt3-L aliquots should be stored at –20^oC for maximal stability up to six months.

l          Avoid repeated freeze-thaw cycles.

Usage

l          FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.