Specifications and Use

Description

l          Recombinant Human Superoxide Dismutase produced in E. coli. is a stable dimer of two identical subunits, non-glycosylated, containing 308 amino acid residues, two pairs of disulfide bonds and having a combined molecular mass of 31.6kD.

Source

l          E. coli.

Molecular Mass

l          Approximately 31.6kD.

Purity

l          ≥95%, as determined by reduced SDS-PAGE

l          Dimer ≥90%, as determined by SEC-HPLC.

 

Endotoxin Level

l          ≤1EU/µg, determined by the LAL method.

Biological Activity

l          ≥7000U/mg

 

Formulation

l          Lyophilized from a 0.2μm filtered solution in 20mmol Phosphate buffer.

 

Reconstitution

l         It is recommended to reconstitute the lyophilized rHuSOD in sterile ddH₂O.

 

Storage

l          Lyophilized samples are stable for greater than three months from date of receipt at -20℃.

l          Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month without detectable loss of activity.