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Recombinant Human Superoxide Dismutase (rHuSOD)

 

 

Specifications and Use

 

Description

l          Recombinant Human Superoxide Dismutase produced in E. coli. is a stable dimer of two identical subunits, non-glycosylated, containing 308 amino acid residues, two pairs of disulfide bonds and having a combined molecular mass of 31.6kD.

 

Source

 

l          E. coli.

Molecular Mass

 

l          Approximately 31.6kD.

 

Purity

 

l          95%, as determined by reduced SDS-PAGE

l          Dimer 90%, as determined by SEC-HPLC.

 

Endotoxin Level

 

l          1EU/µg, determined by the LAL method.

Biological Activity

l          7000U/mg

 

Formulation

 

l          Lyophilized from a 0.2μm filtered solution in 20mmol Phosphate buffer.

 

Reconstitution

l         It is recommended to reconstitute the lyophilized rHuSOD in sterile ddH2O.

 

Storage

 

l          Lyophilized samples are stable for greater than three months from date of receipt at -20.

l          Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8 for one month without detectable loss of activity.

 

 

 

    FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.  

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Last modified: 05/19/16