Description

IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R alpha and gp130. Mouse IL-6 is inactive on human cells, while both human and mouse are equally active on mouse cells. Recombinant human IL-6 is a 20.8 kDa protein containing 184 amino acid residues.

Specifications and Use

Source

l          Escherichia coli.

Molecular Mass

l          20.8kDa

Purity

l          Greater than 95% by SDS-PAGE analyses.

Biological Activity

l          The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6 dependent mouse 7TD1 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 10⁷units/mg.

Endotoxin Level

l          Less than 1EU/µg.

Formulation

l        Sterile filtered lyophilized (freeze-dried) powder.

 

Solubility

l        It is recommended to reconstitute the lyophilized rHuIL-6 in sterile ddH₂O containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 1mg/ml of the cytokine.

 

Stability

l          The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20℃.

l          Reconstituted human IL-6 should be stored in working aliquots at -20℃.

l          Avoid repeated freeze-thaw cycles.

Usage

l          FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.