Specifications and Use

Description

l          Recombinant human IL-4 produced in Yeast is a single, glycosylated, polypeptide chain containing 129 amino acids, three pairs of disulfide bonds and having a molecular mass of approximately 15.0kD (after glycosylation, the molecular mass is 30.0kD.)

Source

l          Yeast

Molecular Mass

l         Approximately 30.0kD.

 

Purity

l          ≥97%, as determined by SDS-PAGE and HPLC method.

Endotoxin Level

l          ≤1EU/µg, determined by the LAL method.

Biological Activity

l       Measured in a cell proliferation assay using a human factor-dependent cell line, TF-1. The specific activity shall be not less than 1×10⁷IU/mg.
 

Formulation

l          Lyophilized from a 0.2μm filtered solution in 20mM Phosphate Buffer.

 

Reconstitution

l          It is recommended that sterile ddH₂O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 5mg/mL of the cytokine.

 

Storage

l          Lyophilized samples are stable for greater than six months from date of receipt at -20℃ to -70℃.

l          Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.

l          Avoid repeated freeze-thaw cycles.