Specifications and Use

Description

l          Recombinant human IL-2 produced in Yeast is a single, non-glycosylated, polypeptide chain containing 133 amino acids, one pair of disulfide bond and with 125 cys. aa. mutated to Ala aa, having a molecular mass of approximately 15.4kD.

Source

l          Yeast

Molecular Mass

l         Approximately 15.4kD.

 

Purity

l          ≥97%, as determined by SDS-PAGE and HPLC method.

Endotoxin Level

l          ≤1EU/µg, determined by the LAL method.

Biological Activity

l         Measured in a cell proliferation assay using an IL-2 dependent murine cytotoxic T cell line, CTLL-2. The specific activity shall be not less than 3×10⁷IU/mg.
 

Formulation

l          Lyophilized from a 0.2μm filtered solution in 10mM Phosphate buffer containing 0.3% human serum albumin.

 

Reconstitution

l          It is recommended that sterile ddH₂O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL.

 

Storage

l          Lyophilized samples are stable for greater than six months from date of receipt at -20℃ to -70℃.

l          Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.

l          Avoid repeated freeze-thaw cycles.