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Recombinant Human Interleukin 1 alpha (rHuIL-1a

 

 

Description

 

IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant human IL-1alpha is an 18.0 kDa protein containing 159 amino acid residues.

 

 

Specifications and Use

 

Source

 

l          Escherichia coli.

Molecular Mass

 

l          18kDa

 

Purity

 

l          Greater than 95% by SDS-PAGE analyses.

Biological Activity

l         The ED50 as determined by the dose-dependent stimulation of mouse D10g4.1 cells is < 0.001 ng/ml, corresponding to a specific activity of > 1 x 109 units/mg.

 

Endotoxin Level

 

l          Less than 1EU/µg.

Formulation

 

l          Sterile filtered lyophilized (freeze-dried) powder (20mM Tris-Hcl,50mM NaCl,pH8.5)  

 

Solubility

l          It is recommended to reconstitute the lyophilized rHuIL-1 alpha in sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 1mg/ml of the cytokine.

 

Stability

 

l          The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20.

l          Reconstituted human IL-1α should be stored in working aliquots at -20.

 

Usage

l          FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.

 

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Last modified: 05/19/16