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Recombinant Human Interleukin 1alpha
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Recombinant Human Interleukin 1 alpha
（rHuIL-1a）
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Description
IL-1alpha is a non-secreted
proinflammatory cytokine produced in a
variety of cells including monocytes, tissue
macrophages, keratinocytes and other
epithelial cells. Both IL-1alpha and
IL-1beta binds to the same receptor and has
similar if not identical biological
properties. These cytokines have a broad
range of activities including, stimulation
of thymocyte proliferation, by inducing IL-2
release, B-cell maturation and
proliferation, mitogenic FGF-like activity
and the ability to stimulate the release of
prostaglandin and collagenase from synovial
cells. However, whereas IL-1beta is a
secreted cytokine, IL-1alpha is
predominantly a cell-associated cytokine.
Recombinant human IL-1alpha is an 18.0 kDa
protein containing 159 amino acid residues.
Specifications
and Use
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Source
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Escherichia coli. |
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Molecular Mass
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18kDa
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Purity
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Greater than 95% by SDS-PAGE analyses. |
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Biological Activity
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The ED50 as determined by the dose-dependent
stimulation of mouse D10g4.1 cells is <
0.001 ng/ml, corresponding to a specific
activity of > 1 x 109 units/mg.
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Endotoxin Level
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Less than 1EU/µg. |
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Formulation
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Sterile filtered lyophilized (freeze-dried)
powder (20mM Tris-Hcl,50mM NaCl,pH8.5)
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Solubility
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It is recommended to reconstitute the
lyophilized rHuIL-1 alpha
in sterile ddH2O containing at
least 0.1% human serum albumin or bovine
serum albumin to prepare a stock solution of
no less than 1mg/ml of the cytokine.
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Stability
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The lyophilized protein is stable for a few
weeks at room temperature, but best stored
at -20℃.
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Reconstituted human IL-1α should be stored
in working aliquots at -20℃.
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Usage |
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FOR RESEARCH USE ONLY. NOT FOR HUMAN USE. |
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Last modified:
05/19/16
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