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Recombinant Human Interleukin 17
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Recombinant Human Interleukin
17 (rHuIL-17)
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Description
The originally described IL-17 protein, now
known as IL-17A, is a homodimer of two 132
amino acid chains, secreted by activated
T-cells that act on stromal cells to induce
production of proinflammatory and
hematopoietic bioactive molecules. Today,
IL-17 represents a family of
structurally-related cytokines that share a
highly conserved C-terminal region but
differ from one another in their N-terminal
regions and in their distinct biological
roles. The six known members of this family,
IL-17A through IL-17F, are secreted as
homodimers. IL-17A exhibits cross-species
bioactivity between human and mouse cells.
Recombinant human IL-17A is a 31.0kDa
disulfide-linked homodimer of two 136 amino
acid polypeptide chains.
Specifications
and Use
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Source
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Escherichia coli. |
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Molecular Mass
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15kDa
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Purity
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Greater than 95% by SDS-PAGE analyses. |
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Biological Activity
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The ED50 as determined by the dose-dependent
induction of IL-6 in Hs68 cell line was
found to be approximately 2ng/ml.
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Endotoxin Level
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Less than 1EU/µg. |
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Formulation
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Sterile filtered lyophilized (freeze-dried)
powder
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Solubility
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It is recommended to
reconstitute the lyophilized rHuIL-17 in
sterile ddH2O containing at least
0.1% human serum albumin or bovine serum
albumin to prepare a stock solution of no
less than 1mg/ml of the cytokine.
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Stability
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The lyophilized protein is stable for a few
weeks at room temperature, but best stored
at -20℃.
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Reconstituted human IL-17 should be stored
in working aliquots at -20℃.
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Avoid repeated freeze-thaw cycles.
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Usage |
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FOR RESEARCH USE ONLY.
NOT FOR HUMAN USE.
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Last modified:
05/19/16
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