Description

IL-15 is an immunomodulating cytokine that stimulates the proliferation of T lymphocytes and shares many biological properties with IL-2. IL-15 exerts its biological activites primarily on T cells. It is also essential in the development, survival and activation of NK cells. Increased expression of IL-15 has been implicated with rheumatoid arthritis, inflammatory bowel disease and diseases affiliated with retroviruses HIV and HTLV-I. Human IL-15 is biologically active on mouse cells as measured by the dose-dependent stimulation of the proliferation of mouse CTLL cells. Recombinant human IL-15 is a 13.2 kDa protein consisting of the following 114 amino acid residues.

Specifications and Use

Source

l          Escherichia coli.

Molecular Mass

l          13.2kDa

Purity

l          Greater than 98% by SDS-PAGE analyses.

Biological Activity

l          Measured in a cell proliferation assay using CTLL-2 cells, the specific activity shall be not less than 2 x 10⁶ units/mg.

Endotoxin Level

l          Less than 1EU/µg.

Formulation

l         Sterile filtered lyophilized (freeze-dried) powder.

 

Solubility

l       It is recommended to reconstitute the lyophilized rHuIL-15 in sterile ddH₂O containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 1mg/ml of the cytokine.

 

Stability

l          The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20℃.

l          Reconstituted human IL-15 should be stored in working aliquots at -20℃.

l          Avoid repeated freeze-thaw cycles.

Usage

FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.