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Recombinant Human GM-CSF (rHuGM-CSF) 

 

 

 

Specifications and Use

 

Description

 

l          Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

 

Source

l          E. coli

 

Molecular Mass

 

l         Approximately 14.5kD.

 

Purity

 

l          97%, as determined by SDS-PAGE and HPLC method.

Endotoxin Level

 

l          1EU/µg, determined by the LAL method.

Biological Activity

l          Measured in a cell proliferation assay using the factor-dependent cell line, TF-1, the specific activity shall be not less than 1×107IU/mg.

 

Formulation

 

l          Lyophilized from a 0.2mm filtered solution in 10mM Phosphate Buffer.

 

Reconstitution

l          It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.

 

Storage

 

l          Lyophilized samples are stable for greater than six months from date of receipt at -20 to -70.

l          Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.

l          Avoid repeated freeze-thaw cycles.

 

  

 

 

    FOR RESEARCH USE ONLY. NOT FOR HUMAN USE.  

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Last modified: 05/19/16