Specifications and Use

Description

l          Recombinant human G-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 18.8kD.

Source

l          E. coli

Molecular Mass

l        Approximately 18.8kD.

 

Purity

l          ≥97%, as determined by SDS-PAGE and HPLC method.

Endotoxin Level

l          ≤1EU/µg, determined by the LAL method.

Biological Activity

l          Measured in a cell proliferation assay using NFS-60 cell line. The specific activity shall be not less than 0.8×10⁸IU/mg.
 

Formulation

l          Lyophilized from a 0.2mm filtered solution of 10mM Acetate Buffer.

 

Reconstitution

l          It is recommended that sterile ddH₂O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.

 

Storage

l          Lyophilized samples are stable for greater than six months from date of receipt at -20℃ to -70℃.

l          Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for up to one month without detectable loss of activity.

l          DO NOT FREEZE AFTER RECONSTITUTION! Loss of activity has been observed upon thawing.