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Recombinant Human Flt3 Ligand
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Recombinant Human Flt3 Ligand
(rHuFlt3-L)
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Description
Flt3-ligand (flk2-ligand) stimulates the
proliferation and colony formation of
certain BM precursor cells including CD34+
cells. Recombinant Human Flt3-Ligand is a
soluble 17.6 kDa polypeptide containing 155
amino acid residues, which comprises the
extracellular domain of the transmembrane
flt3-ligand protein. |
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Specifications
and Use
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Source
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l
Escherichia coli.
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Molecular Mass
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l
17.6kDa |
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Purity
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l
³ 98%
as determined by SDS-PAGE and HPLC analyses.
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Biological Activity
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l
rHuFlt3-L is fully biologically active when
compared to standards. Its specific activity
is
³1×106
units/mg.
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Endotoxin Level
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l
Less than 10EU/mg of rHuFlt3-L as determined
by LAL method.
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Formulation
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l
Sterile filtered lyophilized (freeze-dried)
powder, with 20mM Tris.HCl, 20mM NaCl，pH7.4
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Solubility
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l
It is recommended to reconstitute the
lyophilized rHuFlt3-L in sterile distilled H2O
to prepare a stock solution of no less than
100
mg/ml
of the cytokine.
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Stability |
l
The lyophilized protein is stable for a few
weeks at room temperature, but best stored
at -20℃..
l
Reconstituted rHuFlt3-L should be stored in
working aliquots at -20℃.
l
Avoid repeated freeze-thaw cycles.
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Usage |
l
FOR RESEARCH USE ONLY.
NOT FOR HUMAN USE. |
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Last modified:
05/19/16
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