Quinolones (QNS) ELISA Kit

Brief
Quinolones (QNS) ELISA Test Kit

Principle
This test kit is based on the competitive enzyme immunoassay. The coupling antigen ipre-coated on the micro-well stripes. The Quinolones in the testing sample competes with the coupling antigens pre-coated on the micro-well stripes for the antibodies against Quinolones. After the addition of the enzyme conjugate, the TMB substrate is added forcoloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Quinolones in it. This value is compared to the standard curve and the content of the corresponding Quinolones is subsequently obtained.

Specifications
 

Sensitivity : 0.2 ppb
Cross-reactions
      Enrofloxacin 100%
      Ciprofloxacin 92.88％
      Ofloxacin 98.86％
      Oxolinic Acid 116.86％
      Danofloxacin 102.63％
      Norfloxacin 148.65％
      Lomefloxacin 86.24％
      Pefloxacin 156.60％
      Enoxacin 98.97％
      Flumequine 96.73％
      Marbofloxacin 110.63％
      Amifloxacin 98.86％
      Nadifloxacin 56.81％
      Fleroxacin 38.79％
      Piromidic acid 36.77％
      Levofloxacin 21.36％
      Difloxacin 20.16％
      Sarafloxacin 13.78％
      Nalidixic acid 10.11％
      Cinoxacin 6.40％
      Gatifloxacin 6.12％
      Pipemidic acid 5.23％
      Tosufloxacin 3.16％
      Orbifloxacin 2.23％
      Pazufloxacin 2.17％
      Benofloxacin 1.12％

Recovery rate
       tissue (chichen/liver,pork/liver,shrimp,fish) 80±15%
       serum 80±15%
       honey 75±15%
Detection limit
      Enrofloxacin 3ppb
      Ciprofloxacin 3ppb
      Ofloxacin approx 3ppb
      Danofloxacin approx 3ppb
      Norfloxacin approx 1.5ppb
      Lomefloxacin approx 3ppb
      Pefloxacin approx 1.5ppb
      Enoxacin approx 3ppb
      OxolinicAcid approx 3ppb
      Flumequine approx 3ppb
      Marbofloxacin approx 3ppb
      Amifloxacin approx 3ppb
      Nadifloxacin approx 6ppb