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CERTIFICATE OF ANALYSIS
#100 Cholera Toxin
(Azide-Free)
from Vibrio cholerae
Inaba 569B
Price: 245 Euro
Contents:
Each vial of cholera toxin, when reconstituted to 1.0 ml with sterile distilled water, contains 1.0 mg of protein in: 0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5.
Solubility of cholera toxin, in water, decreases with time, following lyophilization.
Concentration:
Each vial contains 1 mg determined by extinction coefficient of 1.14 at 280 nm and 1 cm for 1 mg/ml.1
Purity:
The preparation migrates as a single major band in disc gel electrophoresis (nondenaturing conditions). The R280/260 of each preparation is reported.
Activity:
When examined in an ADP-ribosylation assay,2,3 the specific enzymatic activity of each lot is at least as high as that of a standard cholera toxin preparation.
Packaging/Storage:
Lyophilized powder stoppered under vacuum. It is recommended that this material be stored at 4°C prior to and following reconstitution. DO NOT FREEZE.
Handling:
Good laboratory technique should be employed in the safe handling of this
product. This requires observing the following practices:
- Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.
- Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.
- Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
- This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or as a diagnostic agent. List Biological Laboratories, Inc. is not liable for any damages resulting from the misuse or handling of this product.
FOR RESEARCH PURPOSES ONLY. NOT FOR HUMAN USE.
References:
- Spangler, Brenda D. (1992) Microbiological Reviews 56 (4), 622-647.
- Tsai, S.-C., Noda, M., Adamik, R., Moss, J., and Vaughan, M. (1987) Proc. Natl. Acad. Sci. USA 84, 5139-5142.
- Tsai, S.-C, Noda, M., Adamik, R., Chang, P.P., Chen, H.-C., Moss, J., and Vaughan, M. (1988) J. Biol. Chem. 263, 1768-1772.
