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PCV ELISA test kit
Brief
The porcine circovirous ELISA Test kit is used for
the detection the porcine circovirus antibody in the serum; assessing the
immunity conditions against porcine circovirus in the pig farm; and for the
investigation of the epidemiology of the porcine circovirus.
The test kit is made from the antigen coated microplate, enzyme conjugate (HRP
goat-anti-pig IgG), and other reagents; it applies the indirect ELISA
principle to test the antibody against O type antibody of porcine circovirus in
the serum.
The test kit is the antigen coated microplate made through the inactivation, purification and concentration of the porcine circovirus. In the test, the diluted contrast serum and sample (to be tested) are added, and incubate; after that, if the sample exists porcine circovirus specific antibody, it will be bound with the antigens of the microplate; eliminate the unbound antibody and other compositions by washing , and adding enzyme conjugate (anti-pig IgG-HRP) to specifically bind with the compound of antibody and antigen of the microplate. Then the unbound conjugate is removed by washing, add the TMB substrate in the well, to react with HRP into a blue product. After discontinuing the reaction by adding HF solution, test the OD value in each reaction well with ELIASA at 630nm wavelength. Specifications
Reagents of the Test Kit
1 Antigen coated microplate......................................................................... 2 pieces (96 wells)
2 Negative control serum (green lid) ........................................................... 1 tube (1ml/tube)
Positive control serum (red lid)................................................................... 1 tube (1ml/tube)
3 Anti-pig IgG-HRP combination(yellow lid)............................................... 1 bottle (22ml/bottle)
4 20×concentrated washing solution (white lid)......................................... 1 bottle (30ml/bottle)
5 Substrate A (red lid) .................................................................................... 1 bottle (12ml/bottle)
Substrate B (black lid).................................................................................. 1 bottle (12ml/bottle)
6 Stop solution (blue lid)................................................................................. 1 bottle (12ml/bottle)
7 Sample diluent solution (transparent lid)................................................... 1 bottle (50ml/bottle)
Storage:
store 2
Expiry date: 6 months
