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Protein Phosphatases

All products below are supplied per vial as indicated in 50 ml 50 mM Tris-HCl, pH 7.0 buffer containing 14 mM b-mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, 50% glycerol. Maintain all preparations at -70°C. Avoid repeated thawing. One unit is the amount of phosphatase that releases 1 nmol of inorganic phosphate from the indicated radiolabeled substrate per min. For ordering information, click here.
PP2A1; Protein Phosphatase 2A1
GLO130 (1 mg, $285)

Divalent cation-independent heterotrimeric protein phosphatase involved in regulating numerous cellular processes including the cell cycle, growth and differentiation. Implicated as growth suppressor and dis-regulation in cancer. Source bovine kidney. Purity > 90% by SDS-PAGE. Subunits ~ 36-, 55-, and 65-kDa. Specific activity ~ 2000 units/mg with MBP as substrate. For MBP, see GLO126, kinase substrates.

ref: 1. Biochem J 287: 1019 2. Proc Natl Acad Sci USA 90: 2500 3. Biochem J 2001 353: 417

PP2A2; Protein Phosphatase 2A2
GLO131 (1 mg, $285)

Dimeric form of PP2A, used also for understanding the function of the missing B subunit of the enzyme. Source bovine kidney. Purity > 90% by SDS-PAGE. Subunits ~ 36- and 65- kDa. Specific activity ~ 2000 units/mg with MBP as substrate. For MBP, see GLO126, kinase substrates.

ref: 1. Biochem J 287: 1019 2. Proc Natl Acad Sci USA 90: 2500 3. Biochem J 2001 353: 417

PP2AC; Protein Phosphatase 2AC
GLO132 (1 mg, $285)

Divalent cation-independent preparation of catalytic subunit of PP2A. Used for functional studies including the role of the missing A (NEW GLO138, GLO139 modulator proteins) and variable B subunits of the enzyme. Source bovine kidney. Purity > 90% by SDS-PAGE. Apparent Mr ~ 36-kDa. Specific activity ~ 2000 units/mg with MBP as substrate. For MBP, see GLO126, kinase substrates.

ref: 1. Biochem J 287: 1019 2. Proc Natl Acad Sci USA 90: 2500 3. Biochem J 2001 353: 417

PP1C; Protein Phosphatase 1C
GLO133 (1 mg, $285)

Growth-factor-stimulated divalent cation-independent protein phosphatase involved in regulating numerous cellular processes including protein synthesis and cell growth. Implicated in cancer and diabetes. In contrast to recombinant forms, rabbit skeletal muscle PP1C is divalent cation-independent. Source bovine brain. Purity > 90% by SDS-PAGE. Apparent Mr ~ 36-kDa. Specific activity ~ 2000 units/mg with phosphorylase as substrate.

ref: 1. Biochem J 287: 1019 2. Proc Natl Acad Sci USA 90: 2500 3. J Biol Chem 273: 27716

PP2B; Protein Phosphatase 2B, Calcineurin
GLO134 (1 mg, $285)

Ca2+/calmodulin activated protein phosphatase involved in regulating numerous cellular processes. Source bovine brain. Native enzyme. Purity > 90% by SDS-PAGE. Subunits ~ 60- and 15-kDa polypeptides. Specific activity ~ 600 units/mg with MBP as substrate. For MBP, see GLO126, kinase substrates.

ref: Biochem J 287: 1019

PP2C; Protein Phosphatase 2C
GLO135 (1 mg, $285)

Mg2+-dependent protein phosphatase involved in regulating numerous cellular processes. Source bovine brain. Purity > 90% by SDS-PAGE. Apparent Mr ~ 45-kDa. Specific activity ~ 1000 units/mg with MBP as substrate. For MBP, see GLO126, kinase substrates.

ref: Biochem J 287: 1019

PDHP; Pyruvate Dehydrogenase Phosphatase
GLO136 (1 mg, $285)

Insulin-activated Mg2+-dependent and Ca2+-stimulated mitochondrial protein phosphatase specific for the pyruvate dehydrogenase complex (PDC). Source bovine kidney. Purity > 90% by SDS-PAGE. Subunits ~ 50-kDa catalytic and 100-kDa regulatory, which modulates Mg2+ sensitivity. Specific activity ~ 900 units/mg with PDC as substrate. Supplied in 50 ml 50 mM Tris-HCl, pH 7.0 buffer containing 14 mM b-mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, 0.05 mg/ml bovine serum albumin and 50% glycerol. For PDC, see GLO124, kinase substrates.

ref: 1. Biochemistry 21: 5585 2. Biochem Biophys Res Commun 124: 95 3. Biochem. J 238: 83 4. Proc Natl Acad Sci USA 93: 4953

 

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