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Nitrofuran (SEM) ELISA Kit
Brief
SEM ELISA kit, Semicarbazide (SEM) ELISA Test Kit
Principle
This test kit is based on the competitive enzyme
immunoassay for the detection of semicarbazide (SEM) in the tissue(chicken,
fish, shrimp), milk, honey, whole egg. The coupling antigen is pre-coated on the
micro-well stripes. The SEM in the testing sample competes with the coupling
antigen pre-coated on the micro-well stripes for the antibody against SEM. After
the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the testing sample is inversely proportional
to the SEM in the sample. This value is compared to the standard curve and the
content of the corresponding SEM is subsequently obtained.
Specifications
Sensitivity : 0.1ppb
Detection limit:
Tissue ,honey ,egg 0.2 ppb
Fish, shrimp(some interference in shrimp and fish) 0.3 ppb
Recovery rate
Shrimp and fish 85±10%
Honey ,chicken meat /liver,whole egg 75±15%
Cross-reaction rate:
SEM 100%
AHD ﹤0.1%
AMOZ ﹤0.1%
AOZ ﹤0.1%
