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Nitrofuran (AOZ) ELISA Kit
Brief
Nitrofuran (AOZ) ELISA Test Kit
Principle
This test kit is based on the competitive enzyme
immunoassay for the detection of AOZ (3-anmino-2-oxazolidinone) in the
tissue(chicken, fish,shrimp). The coupling antigen is pre-coated on the
micro-well stripes. The AOZ in the testing sample competes with the coupling
antigen pre-coated on the micro-well stripes for the antibody against AOZ. After
the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the testing sample has a negative correlation
with the content of AOZ in it. This value is compared to the standard curve and
the content of the corresponding AOZ is subsequently obtained.
Specifications
Sensitivity : 0.05ppb
Detection limit:
Tissue and honey 0.1ppb
Shrimp, fish(some interference in shrimp and fish) 0.2ppb
Recovery rate
Shrimp and fish 90±10%
Honey, chicken, meat/liver 75±15%
Cross-reaction rate:
AOZ 100%
AHD ﹤0.1%
AMOZ ﹤0.1%
SEM ﹤0.1%
