Nitrofuran (AMOZ) ELISA Kit

Brief
Nitrofuran (AMOZ) ELISA Test Kit

Principle
This test kit is based on the competitive enzyme immunoassay for the detection of AMOZ (3-anmino-5-morpholinomethyl) in the tissue (chicken, fish,shrimp). The coupling antigen is pre-coated on the micro-well stripes. The AMOZ in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibody against AMOZ. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of AMOZ in it. This value is compared to the standard curve and the content of the corresponding  AMOZ is subsequently obtained.

Specifications
 

Specification: 96wells /kit

Sensitivity: 0.1 ppb

Detection limit

Fish/shrimp (some interference in shrimp and fish)0.3 ppb

Tissue and honey0.2 ppb

Recovery rate

Shrimp and fish90±15%

Honey, chicken/liver80±15%

Cross-reaction rate

AMOZ100%

AHD﹤0.1%

AOZ﹤0.1%

SEM﹤0.1%