GENTAUR Contact Gentaur

GENTAUR Survey

GENTAUR EUROPE

+32 1658 9045
+32 1650 9045

BELGIUM1

tel +32 2 732 5688
fax +32 2 732 4414
info@gentaur.com
Av. de l' Armée 68
B-1040 Brussels

France

tel 01 43 25 01 50
fax 01 43 25 01 60
9, rue Lagrange
75005 Paris

Italy

tel 02 36 00 65 93
fax 02 36 00 65 94
20135 Milano

Germany

tel +32 16 58 90 45
fax +32 16 50 90 45
Forckenbeckstraße 6
D-52074 Aachen

Japan

tel +81 78 386 0860
fax +81 78 306 0296
Minaatojimaminami-manchi
Chuo-ku, Kobe
065-0047


SpinClean TM Chelating Excellose® Kit


Introduction

Chelating Excellose® kit provides kit provides fast and one step method for small-scale purification of 6xHis tagged protein. It uses IDA Excellose® spin column containing resin with immobilized Ni2+ ion and centrifugation for rapid processing of multiple samples.
 

How to use

Chelating Excellose® kit contains Ni2+ charged IDA Excellose®, column, collectionTube, and stock solution.
Protocols
1. Vortex the IDA Excellose tube and pipet 0.5ml of the slurry to Column, add 500 ul of equilibration buffer, and centrifuge at 700 x g (approx. 2,000 rpm) for 2 min.
2. Discard the eluate, load up to 0.5 ml of cell lysate, centrifuge for 2 min, and collect the flow-through fraction.
3. Add 0.5 ml of Washing Buffer, centrifuge for 2 min, and collect the wash fraction.
4. Repeat step 3, if necessary.
5. Add 0.2 ml of Elution Buffer and centrifuge.
6. Collect the eluate.
 

Ordering Information


 

Catalog No
Package
Price ($)
22020
columns/pkg
 
22050
columns/pkg
 



 

6xHis tagged protein purification
E. coli lysate containing 6xHis tagged
protein was loaded to IDA Excellose® spin column.

M. Molecular weight marker
1. E. coli cell lysate
2. Flow-through
3. Eluate
 

send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2008 Gentaur Molecular Products
Site powered by Acid Dragon (AC)
Last modified: 05/19/16