Description:
- Up to 6 fold yield increase compared to other baculovirus
expression systems
- Improved protein quality through enhanced disulfide bond formation
- Higher recombination efficiency
Sapphire™ Baculovirus DNA was specifically designed for efficient
recombination with a polyhedrin promoter-based baculovirus transfer
vector. The Sapphire DNA contains a lethal deletion of ORF1629, which
can only result in viable viral particles if rescued by homologous
recombination with a polyhedrin promoter. This design leads to virtually
100% recombination efficiency and significantly simplifies
identification of recombinant virus. In addition, the disulfide
isomerases gene was inserted at the p10 locus of the virus to ensure
proper protein disulfide bond formation. The Sapphire™ Baculovirus DNA
is compatible with most of the commercial polyhedrin-based transfer
vectors.
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