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NEW PRODUCTS

 
BCKDH Kinase Kit NEW!
GLO125-01 ($385)

Effective for determining the activity of BCKDH Kinase and to evaluate the effects of test substances on its activity. Kit provides for 50 assays. Assay is based on the reaction of the kinase with BCKDH complex and ATP. Kit includes directional insert, and contains solutions of Mg , ATP, assay buffer, and purified sample of BCKDH complex. Store solutions at -70°C. Avoid repeated thawing. g-32P-ATP and trichloroacetic acid are not provided with kit. For BCKDH complex, see GLO125, kinase substrates).


ref: Methods Enzymol 166: 321

DEK oncoprotein NEW!
GLO142 (1 mg, $320)

DEK is a nuclear phosphoprotein of apparent Mr ~ 43 kDa that is fused to the nucleoporin CAN as a result of a recurrent (6;9) chromosomal translocation in a subset of acute myeloid leukemias and myelodysplastic syndrome. DEK has also been identified as an autoimmune antigen in juvenile rheumatoid arthritis and other rheumatic diseases. DEK changes the topology of DNA by introducing constrained positive supercoils into protein-free and chromatin-associated DNA. DEK also associates with the transcriptional co-repressor Human Daxx, the SRm160 splicing coactivator, and the HIV-2 enhancer. DEK exhibits some structural similarity to I2aPP2A (GLO141, modulator proteins), which also occurs as a fusion protein with the nucleoporin CAN in acute undifferentiated leukemia. However, whether DEK and I2aPP2A act via similar mechanisms in vivo is currently unknown.

GloboZymes provides GST-tagged recombinant human preparations of DEK. Purity > 90% by SDS-PAGE. Apparent Mr ~ 70-kDa. Supplied 50 ml in 50 mM Tis-HCl, pH 7.0 buffer containing 0.1 mM phenylmethanesulfonyl fluoride, 14 mM b-mercaptoethanol, 1 mM EDTA and 10% glycerol. Maintain in aliquots at -70°C. Avoid repeated thawing.

ref: 1. Mol Cell Biol 12: 1687 2. Clin Exp Immunol 94: 435 3. Proc Natl Acad Sci USA 94: 1811 4. J Cell Sci 115: 3319

Subunit A of Protein Phosphatase 2A NEW!
GLO138 (1 mg, $310)

The A (also termed PR65) subunit of PP2A (GLO130-GLO132, protein phosphatases) serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit, generating functionally diverse heterotrimers of the phosphatase. The A subunit also serves to regulate the polycation response of the phosphatase. Mutations of the A subunit are associated with lung and colon tumors. Preparations of the A subunit are useful for further studies on its function. Source recombinant human protein. Purity > 90% by SDS-PAGE. Apparent Mr ~ 65-kDa. Supplied 50 ml in 50 mM Tis-HCl, pH 7.0 buffer containing 0.1 mM phenylmethanesulfonyl fluoride, 14 mM b-mercaptoethanol, 1 mM EDTA and 50% glycerol. Maintain in aliquots at -70°C. Avoid repeated thawing.

ref: 1. Eur J Biochem 267: 5209 2. Eur J Biochem 248: 200

AGlu416; Mutant A subunit of protein phosphatase 2A NEW!
GLO139 (1 mg, $310)
AGlu416 contains a mutation in a basic motif that is an important determinant for the interaction of the A and catalytic subunits of PP2A (GLO130-GLO132, protein phosphatases). The AGlu416 preparations can be useful as control in reconstitution studies and for further delineating the functional significance of the basic motif. Source recombinant human protein. Purity > 90% by SDS-PAGE. Apparent Mr ~ 65-kDa. Supplied 50 ml in 50 mM Tis-HCl, pH 7.0 buffer containing 0.1 mM phenylmethanesulfonyl fluoride, 14 mM b-mercaptoethanol, 1 mM EDTA and 50% glycerol. Maintain in aliquots at -70°C. Avoid repeated thawing.

ref: 1. Eur J Biochem 267: 5209 2. Eur J Biochem 248: 200 3. Cell 96: 99

GST-I1; Protein phosphatase 1 inhibitor NEW!
GLO137 (1 mg, $285)

GST-tagged form of potent inhibitor of PP1 (see GLO133, protein phosphatases) which is activated following phosphorylation by protein kinase A. The GST tagged form is effective for pull down assays. Source recombinant human protein. Purity > 90% by SDS-PAGE. Apparent Mr ~ 50-kDa. Supplied 50 ml in 50 mM Tis-HCl, pH 7.0 buffer containing 0.1 mM phenylmethanesulfonyl fluoride, 14 mM b-mercaptoethanol and 10% glycerol. Maintain in aliquots at -70°C. Avoid repeated freeze thaw cycles.

ref: J Biol Chem 273: 27716

cPK assay kit NEW!
GLO117-01 ($385)

Useful for determining the activity of cPK (GLO117, protein kinases) or related protein serine/threonine kinase. Can be used to evaluate the effect of a test substance on cPK activity or to identify potential new physiological substrates of the enzyme. Kit provides for 50 assays. Assay is based on the reaction of cPK with protamine sulfate and g-32P-ATP. Kit includes directional insert, and contains solutions of MgATP, protamine sulfate, assay buffer, and purified cPK sample. Store solutions at -70°C. Avoid repeated thawing. g-32P-ATP and trichloroacetic acid are not provided with kit.

Ref: J Biol Chem 264: 6412

MBPK assay kit NEW!
GLO113-01 ($385)

Useful for determining the activity of MBPK-1, MBPK-2 (GLO113, GLO114 protein kinases) or related protein serine/threonine kinase. Can be used to evaluate the effect of a test substance on MBPK-1 activity or to identify new physiological substrates of the enzymes. Kit provides for 50 assays. Assay is based on the reaction of enzyme sample with MBP (GLO126, kinase substrates) and g-32P-ATP. Kit includes directional insert, and contains solutions of MgATP, MBP, assay buffer, and purified MBPK-1 sample. Store solutions at -70°C. Avoid repeated thawing. g-32-ATP and trichloroacetic acid are not provided with kit.

ref: J Biol Chem 268: 15298

CK-2 assay kit NEW!
GLO112-01 ($385)

Useful for determining the activity of CK-2 (GLO112, protein kinases) or related protein serine kinase. Can be used to evaluate the effect of a test substance on CK-2 activity or to identify new physiological substrates of the enzyme. Kit provides for 50 assays. Assay is based on the reaction of CK-2 with casein and g-32P-ATP. Kit includes directional insert, and contains solutions of MgATP, casein, assay buffer, and purified CK-2 sample. Store solutions at -70°C. Avoid repeated thawing. g-32P-ATP and trichloroacetic acid are not provided with kit.

ref: 1. Biochem Biophys Res Commun 166: 449 2. Arch Biochem Biophys 262: 574

AK assay kit NEW!
GLO111-01 ($385)

Useful for determining the activity of AK (GLO111, protein kinases) or related protein serine kinase. Can be use to evaluate the effect of a test substance on AK activity or to identify new physiological substrates of the enzyme. Kit provides for 50 assays. Assay is based on the reaction of AK with MBP (GLO126, kinase substrates) and g-32P-ATP. Kit includes directional insert, and contains solutions of MgATP, MBP, assay buffer, and purified AK sample. Store solutions at -70°C. Avoid repeated thawing. g-32P-ATP and trichloroacetic acid are not provided with kit.

ref: 1. Proc Natl Acad Sci USA 90: 2500 2. J Biol Chem 268: 11193 3. J Cell Physiol 178: 397 4. J Biol Chem 276: 14909

Anti-4E detection kit NEW!
GLO153-01 ($385)

Same as GLO153 (antibodies), except that, an aliquot of eIF-4E is provided in SDS sample buffer ready for use in five applications as positive control in Western blots.

Anti-PP2A detection kit NEW!
GLO148-01 ($385)

Same as GLO148 (antibodies), except that, an aliquot of PP2A is provided in SDS sample buffer ready for use in five applications as positive control in Western blots.

Anti-AK detection kit NEW!
GLO151-01 ($385)

Same as GLO151 (antibodies), except that, an aliquot of an AK preparation is provided in SDS sample buffer ready for use in five applications as positive control in Western blots.

Anti-p65PAK detection kit NEW!
GLO152-01 ($385)

Same as GLO152 (antibodies), except that, an aliquot of p65PAK is provided in SDS sample buffer ready for use in five applications as positive control in Western blots.

Anti-I2a detection kit NEW!
GLO150-01 ($385)

Same as GLO150 (antibodies), except that, an aliquot of I2aPP2A is provided in SDS sample buffer ready for use in five applications as positive control in Western blots.

Anti-I1a detection kit NEW!
GLO149-01 ($385)

Same as GLO149 (antibodies), except that, an aliquot of I1aPP2A is provided in SDS sample buffer ready for use in five applications as positive control in Western blots.
 

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