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Specification sheet |
Human
TNF- α
ELISA Kit
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Catalog No:
OCK001-S1 |
One plate; Stripe; 96well determinations |
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Catalog No:
OCK001-C3 |
Three plate;
Complete; 288well determinations |
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Application |
Human TNF-α
is a capture ELISA for the quantitative measurement of
human TNF-α
in culture media and serum
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Sensitivity |
2pg/ml |
Range of Detection |
15-1500pg/ml |
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Specificity |
Native and recombinant human
TNF-α |
Storage |
Store at 2-8oC |
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Assay time |
Total assay time is 5 - 6hrs at room temperature |
Stability |
5 months when stored at 4oC |
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1.anti-hTNF-α precoated microplate |
2.Lyophilized rhTNF-a
standard |
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3.Streptavidin-HRP concentrate |
4.Biotinylated antibody reagent |
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5.Streptavidin-HRP dilution buffer |
6.Stop solution, 0.18M phosphoric acid |
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7.Premixed TMB substrate solution
A |
8.Premixed TMB substrate solution
B |
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9.Wash buffer 30-fold concentrated |
10.Adhesive plate covers |
11.Standard diluent |
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Protocol ( For Research
Use Only )
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1. Dispense 100µl
of the
standards, controls, and samples into their designated
wells. |
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2. Seal the plate and incubate for 2hours at the room
temperature. |
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3. Wash the plate 3 times with
Wash buffer. |
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4. Add 100µl
of the diluted
Biotinylated- antibody to each
well. |
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5. Seal the plate and incubate it for 2hours at the room
temperature. |
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6. Wash the plate 3 times with
wash buffer. |
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7. Add 100µl
of the diluted Streptavidin-HRP conjugate to each well. |
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8. Seal the plate and incubate it for 30min at the room
temperature. |
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9. Wash the plate more than
4 times with
wash buffer. |
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10. Immediately dispense 100µl
TMB substrate solution (TMB A + TMB B) into each
well and
incubate at room temperature for 15-30mins. |
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11. Add 50µl
of Stop Solution to each well. |
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12. Read absorbance with a microplate reader at 450nm. |
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Reference
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Avirutnan, P., P. Malasit, B. Seliger, S. Bhakdi, and M.
Husmann (1998) Dengue virus infection
of human endothelial cells leads to chemokine
production, complement activation, and apoptosis.
J.Immunol 161(11):6338-46. |
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Aziz, N., et al.
(1999)
Stability of Plasma Levels of Cytokines and
Soluble Activation Markers in Patients with Human
Immunodeficiency Virus Infection. Journal of Infectious
Disease. 179:843-8. |
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Bienvenu, J., et al.
(1993)
Analytical performances of commercial ELISA kits
for IL-2, IL-6 and TNF-alpha.
A WHO study.
Eur. Cytokine Netw.
4(6);447-451. |
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Oliver, S.J., A. Moreira, and G. Kaplan (2000) Immune
stimulation in scleroderma patients treated with
thalidomide. Clinical Immunology 97(2):1980120.
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Last modified:
05/19/16
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