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Culture plate for measuring the resorption ability of osteoclasts
 

 

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SEM picture

SEM picture ( x3,500 )

Ca-P thin film-coated surface

 arrow head: resorption pit /arrow: osteoclast

> Description
Osteoclast is a large multinucleate cell formed from differentiated macrophage, responsible for breakdown of bone. It could be characterized by the multi-nucleation, TRAP positive and formation of resorption pit.
OAASTM is a culture plate to measure the activity of the osteoclast in bone  research.  Due to its bottom coated with low crystalline of carbonated calcium phosphate, OAASTM resembles human bone and will be a perfect substitute for dentin slice ever.
 

> Advantage of OAASTM

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Activities are observed with bare eyes during its cultivation

 
You will have reliable data because calcium phosphate is coated as the substrate for osteoclast, and it is more comparable to human bone than the other product coated with synthetic hydroxyapatite.
While you culture the osteoclast precursor cell, you may observe the differentiation.

(Culture period: 2-3days for matured osteoclast, 7-12 days for precursor cell
)
After removing the matured osteoclasts by NaOCl (sodium hypochlorite), you may measure the resorption pit margin by an image analyzer.
Due to clear calcium carbonate coated, you may observe the resorption area under light microscopy without SEM and even with your bare eyes during culture.
OAASTM can be used for TRAP staining of osteoclasts in situ.
 
Figure 1, 2: Resorption areas in a culture dish after removing osteoclasts
Figure 3: TRAP staining of osteoclasts in situ and resorption area
Figure 4: SEM picture of a osteoclast and resorption area
   
> Specification
Category

 

Description
Product type

 

Culture Plate

 

Growth surface

 

Useful for tissue culture

 

Coating type

 

Low crystalline of carbonated calcium phosphate

 

Lid

 

Yes
Shape

 

Flat-bottom

 

Size

 

4well, 24well, 48well

 

Volume

 

OAAS-4

 

4well

 

2ml

 

OAAS-24

 

24well

 

2ml

 

OAAS-48

 

48well

 

1ml

 

Growth Area

 

OAAS-4

 

4well

 

1.9cm2

 

OAAS-24 24well

 

1.9cm2

 

OAAS-48

 

48well 1cm2

 

Material  White with clear bottom

 

Trademark OAAS

 

Special Order Special order such as trace elements incorporated, different crystallinity, variable thickness, specific protein bound, 96-well plate, or chamber slide / cover slips, may be accepted. Please contact us for further information.
   
How to use OAAS
Image Analysis for OAAS
Papers where OAAS is used
 

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Last modified: 05/19/16