High sensitive fluorescence staining
- Short and easy procedure which gives stronger fluorescence compared to
FITC labelled antibodies or other Fluorescence techniques.
Fungiflora Y TM Kit contains:
Solution A: Haematoxilin as counterstaining solution
Solution B: Fungiflora high sensitive dye as Fluorescence staining
solution
References:
Inoue, T., Asari, S., Tahara, K., Kiritoshi, A.,
Inoue, Y. & Shimomura, Y. Utility of Fungiflora Y stain in rapid diagnosis of
Acanthamoeba keratitis. Br. J. Ophthalmol. 83:632-633, 1999.
Key Words: Acanthamoeba, fungus, Fungiflora Y stain, Fungalase, Parker
ink-potassium hydroxide stain, Fluorescence Microscope, FITC, ImmunoHistoWax
- Direct microscopic examination of clinical materials is needed for
rapid diagnosis of Acanthamoeba keratitis and keratomycosis in the region of
ophthalmology, but complex skills are necessary to confirm the
microorganisms. We compared the Fungiflora Y stain that can dye specifically
Acanthamoeba cyst and fungi, with the rapid-GMS stain, Gram stain, Giemsa
stain, methylen blue stain, direct wet mount non-stain, Parker ink stain,
Fungalase and Parker ink-potassium hydroxide stain. We evaluated a
simultaneous staining method for trophozoite, cyst and fungi, and the
influence of potassium hydroxide against the morphologic change of
Acanthamoeba.
1. The most useful staining method to confirm trophozoites was the methylen blue
stain after trophozoites were fixed to the slide by the Schaudinn solution.
Because this method is completed in a short time, it easily reveals the
acanthopodia and cytoplasm.
2. The most useful staining method to confirm cysts and fungi was the Fungiflora
Y stain because no complex skill is needed.
3. A combination method of methylen blue stain and the Fungiflora Y stain was
useful for simultaneous staining to confirm trophozoites, cysts, and fungi after
fixation using the Schaudinn solution.
4. The Parker ink-potassium hydroxide stain method was not suitable for
detection trophozoite, because trophozoites were broken by potassium hydroxide.
Pneumocentesis liquid, Fungiflora Y staining
(Y.
Higashiyama)