Biobasic´s silica-gel membrane based kits are available in two different formats, either packaged as individual spin columns or in 96-well format for high throughput purifications.

Binding Solution

Wash Solution

Elution Buffer

UNIQ-10 Column

2.0 ml Collection Tube

Protocol

96-Well Plates

Deep Well Collection Plates

Binding Solution

Wash Solution

Elution Buffer

Complete Protocol

Principle:

UNIQ-10 spin columns and 96-well plates utilize silica-gel membrane technology adsorbing selectively up to 10 µg PCR DNA or DNA fragments in the presence of specialized binding buffers. Nucleotides, enzymes, mineral oil and other impurities do not bind to the membrane. PCR products or DNA fragments can be eluted with elution buffer in small volume.

Application:

• Recovery of PCR products from PCR reaction mixture.
• Removal of nucleotides and primers (<50-mers).

Features:

• Rapid and economical. Entire procedure takes 15-20 minutes (UNIQ-10) resp. 40 minutes (UNIQ-96 well).
• High yields (60-90%). Recovers 60 bp - 40 kb DNA fragments.
• Efficient removal of contaminants. Purified DNA can be used in any downstream applications such as sequencing, labeling, restriction enzymatic digestions, ligations, hybridizations or transformations.
• No phenol / chloroform extraction or ethanol precipitation.

Procedure:

The kit uses a simple bind-wash-elution procedure. PCR products are mixed with the appropriate binding buffer and pass through to the column. DNA binds to the silica-based membrane. Impurities such as enzymes, salts, nucleotides and primers ( < 50-mer) are washed away. Pure DNA is eluted in a small volume of the low-salt elution buffer or water, ready for use in any downstream applications.