|
Tsg is supplied with
10 x reaction buffer and 15 mM magnesium chloride.
Tsg is a thermostable DNA Polymerase isolated from a strain of
Thermus sp. It is designed for use in primer extension reaction.
Tsg has a half life of 3 hours at 95°C, it is therefore more
stable than Taq DNA polymerase.
Tsg has higher fidelity than Taq with an error frequency 10e-5 to
10e-6 during DNA synthesis.
|
Order
Information: |
Cat. No.: |
Size |
Price |
D0081-200 |
200 U |
Euro 39 |
|
5 x 200 U |
Euro 119 |
D0081-1000 |
1,000 U |
Euro 119 |
|
5 x 1,000 U |
Euro 399 |
|
Tsg is highly
purified. No detectable contaminating endonuclease, exonuclease and
nicking activity is observed.
Primer Extension
Characteristics: Tsg has the template-independent terminal
transferase activity which results in the addition of a single
nucleotide (adenosine) at 3' end of extension product. So TA cloning
vector is recommended if the extension product is needed to be cloned.
Quality Testing: For
endonuclease assay, 1 µg of Lambda-HindIII DNA is incubated with 20
units of the enzyme in assay buffer at 75°C for 16 hrs. and no visible
contaminating activity is observed. For exonuclease assay, 1 µg of
pBR322 plasmid DNA is incubated with 10 units of the enzyme for 16 hrs
at 75°C in assay buffer and no detectable activity is observed.
Moreover, the purity of the enzyme is also detectable by adding 10 units
of Tsg DNA Polymerase in 0.1ml buffer of a reaction mixture for making
first strand cDNA at beginning and no impaired effect on the first
strand cDNA is observed.
Unit Definition: One
unit incorporates 10 nmol of dNTP into acid-insoluble material in 30 min
at 74°C.
Concentration in Storage
Buffer: 5 units / µl in 20mM Tris HCl (pH 8.0), 0.1mM EDTA, 1mM DTT,
0.1% Triton X-100 and 50% glycerol.
Magnesium Chloride:
The final magnesium chloride may be variable to individual requirements.
In general, 1.5mM magnesium chloride in reaction mixture is workable.
Storage: -20° C
|
|
send mail to [email protected] with questions or comments about this web site.
Copyright © 2008 Gentaur Molecular Products
Site powered by Acid Dragon (AC)
Last modified:
05/19/16
|