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Sample Preparation |
Optimal sample
preparation is crucial for any proteome analysis. The following
parameters define the quality of each proteomics approach:
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Solubilization protocol: Our partner Fraunhofer IGB can either apply
an in house optimized SDS solubilization protocol or depending on
the project other solubilization protocols adapted to the range of
proteins which needs to be analyzed.
- Cell
fractionation: Whenever possible, cell fractions should be used to
reduce the number of total proteins in the 2 compared samples. This
increases the probability of identifying proteome differences.
- Choice of
electrophoretical separation: Depending on the biological question
and on the number of proteins in the prepared samples, a protein
labeling and/or biochemical fractionation strategy with subsequent 1
D protein gel electrophoresis or a 2D protein gel electrophoresis
strategy might be selected.
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Ordering information:
Standard Sample
Preparation:
- For each
protein extract provided by you, the protein amount will be
determined by Bradford assay and the proteins will be solubilized in
an appropriate buffer for gel loading.
- Please send
your protein samples on dry ice to BioCat GmbH, Technologiepark,
Container, Im Neuenheimer Feld 581, 69120 Heidelberg.
- Please
enclose a detailed description how you prepared the samples.
Cat. No.:
SSP01: Standard Sample Preparation for 2D Gel Electrophoresis; 250 €
for 2 samples
Cat. No.:
SSP02: Standard Sample Preparation for 1D Gel Electrophoresis; 125 €
for up to 8 samples
Design of
Sample Preparation:
If you prefer to
supply biological samples rather than protein extracts, please let
us know their source and the biological questions you like to answer
by the proteomics approach. Our partner Fraunhofer IGB will optimize
the proteomics strategy by fractionating the cells or biochemical
labeling if applicable, by choosing the most appropriate
solubilization protocol, and by selecting either 1 dimensional or 2
dimensional electrophoresis as method of choice. Price depends on
the project details. Please ask
for a quotation.
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Last modified:
05/19/16
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