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Mouse Cytokine Microarray II (Fluorescent)
M
ouse cytokine microarray is a protein array kitdesigned to screen diverse mouse samples, such as cell
lysates, serum, plasma and tissue culture supernatants,
for the presence of 14 different cytokines
simultaneously. Each slide in the kit contains eight
identical sub-arrays of 14 capture antibodies in
quadruplicate and supports the analysis of up to eight
40-µL samples. A cocktail of biotinylated detection
antibodies is provided. The antibody pairs bind the
cytokine antigen in a sandwich format. Cytokine
detection is achieved with the addition of streptavidinfluorophore
conjugate. Positive and negative controls
spotted within each sub-array allow for assay
validation. In addition, two-color detection is allowed
when samples are labeled with fluorophores.
Features and Benefits
•
Eliminate blocking step and ready for use•
Screen 14 cytokines simultaneously in one mousesample
•
Process up to eight samples on one slide•
Accept low sample volume (40 µL) per sub-array•
Compatible with diverse sample types•
Allow two-color detection (Cy3 & Cy5)•
Comparable with ELISA’s sensitivity, specificityand dynamic range
•
Fully customizable arrays upon experimentalneeds
M
ouse Cytokines DetectedIL-1â
IL-2
IL-4
IL-5
IL-6
IL-10
IL-13
GM-CSF
IFN-
γRANTES
MCP-1
TNF-
αVEGF
IL-12 (p70)
Microarray Image
Mixed recombinant proteins (IL-1
β, IL-4, IL-6 and GM-CSF)were applied to each sub-array at different concentrations.
PC-positive control; NC-negative control.
Assay Performance of Mouse Cytokine Microarray