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HMGB1 ELISA Instructions
Method / Technology ELISA
Kit size 8x12
Specimen / Volumes 200µL serum, plasma, cell culture supernatant
Standard range 0-116.8 ng/mL
Incubation time 1 x 18h, 1 x 2h, 1 x 30min.
Substrate / Isotope TMB 450nm

HMGB1 - High Mobility Group Box1 Protein
HMGB1 is a protein that is regularly present in the nucleus and cytoplasm of mammalian cells. As a nucleus-binding protein it regulates gene transcription.
HMGB1 plays an important role in diesease pathogenesis, such as malignant diseases. In the case that a tumor is cut off the oxygen supply and it's cells start to die, HMGB1 will be released. In the adjacent environment of the tumor this released HMGB1 causes angiogenetic blood vessel formation towards the tumor again, which will assure the tumor survival and further growth.
In vitro studies have shown, that via quantitative determination of HMGB1 concentrations it is possible to differentiate between pathological cell death (necrosis) and physiological cell death (apoptosis), as you can observe much higher HMGB1 values in necrosis.
HMGB1 also plays an important role in the late phase of septic shock. It was shown that the inhibition of HMGB1 in animal experiments even in the late phase of septic shock significantly enhanced the survival rate of rodents. HMGB1 acts upon binding to RAGE ("receptor for advanced glucated end products") and has a similar proinflammatory effect as the cytokines TNF-α and IL-6. Elevated values can also be found in the serum of septic patients.

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