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SpinClean |
TM |
Glutathione Excellose® Kit |
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Introduction
The Glutathione
Excellose® kit provides fast and one step method for small-scale purification of
GST fusion protein. It uses ready-to-use Glutathione Excellose® spin cloumn
containing and centrifugation for rapid processing of multiple samples.
How to use
Glutathione
Excellose® kit contains Glutathione Excellose®,
spin column, collectionTube, and stock solution.
Protocols
1. Vortex the Glutathione Excellose tube and pipet 0.5ml of the slurry to
Spin Column, add 0.5 ml of Equilibration Buffer in the Spin Column, and
centrifuge at 700 x g (approx. 2,000 rpm) for 2 min.
2. Discard the eluate, load up to 0.5 ml of cell lysate, centrifuge,
and collect the flow-through fraction.
3. Add 0.5 ml of Washing Buffer, centrifuge, and collect the wash fraction.
4. Repeat step 3, if necessary.
5. Add 0.2 ml of Elution Buffer and centrifuge.
6. Collect the eluate
Ordering Information
Catalog No |
Package
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Price ($)
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21020 |
20 columns/pkg
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21050 |
50 columns/pkg
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GST fusion protein purification
E.coli lysate containing GST fusion protein was loaded to Glutathione
Excellose® spin column.1 : E. coli lysate
2 : flow-through fraction
3 : wash fraction
4 : purified GST
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Last modified:
05/19/16
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