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FMD IgG Distinguishing ELISA kit
Brief
Swine Foot and Mouth Disease (FMD)
IgG Distinguishing ELISA Test kit is used for detection the non-structural 3ABC-protein
of swine foot and mouth disease (FMD-3ABC) antibodies in the serum,
differentiation naturally infected from vaccined swine.
The test kit is made from the antigen coated microplate, enzyme conjugate(HRP
goat-anti-pig IgG), and other reagents, it applies the indirect
ELISA principle to test the antibodies against
FMD-3ABC in the serum.
Principle
The test kit is the antigens coated microplate made through FMD-3ABC protein. In the test, the diluted control serum and sample are added, then incubate. If FMD-3ABC specific antibodies exist in the sample, it will be bound with the antigens of the microplate but the unbound antibodies and other components will be removed by washing. Next add enzyme conjugate (anti-pig IgG-HRP) to specifically bind with the compound of antibodies and antigens on the microplate. Then the unbound conjugates will be removed by washing, Add the TMB substrate in the well, react with HRP to a blue product. At last, end the reaction by adding stop solution.
Specifications
Reagents of the Test Kit
1 Antigen coated microplate........................................................................... 2 pieces (96 well)
2 Negative control serum (green lid) ............................................................. 1 tube (1ml/tube)
Positive control serum(red lid)........................................................................ 1 tube (1ml/tube)
3 Anti-pig IgG-HRP combination(yellow lid)................................................ 1 bottle (22ml/bottle)
4 20× concentrated washing solution (white lid).......................................... 1 bottle (30ml/bottle)
5 Substrate A (red lid) ...................................................................................... 1 bottle (12ml/bottle)
Substrate B (black lid)........................................................................................1 bottle (12ml/bottle)
6 Stop solution (blue lid).................................................................................. 1 bottle (12ml/bottle)
7 Sample diluent solution (transparent lid).................................................... 1 bottle (50ml/bottle)
Storage:
store 2
Expiry date: 6 months
