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| Broth™ is a proprietary medium formulation
developed by AthenaES™ for improved expression of
recombinant proteins in E. coli. This medium is
derived from the Superior Broth™ formula but made without
animal-derived materials. The medium is a complex,
rich formulation which supplies a nitrogen source, vitamins,
and moderate glucose levels. The medium is buffered at
pH 7.2±0.2 to prevent acidification of the medium during
metabolism of the glucose. For expression of
recombinant proteins, induction should be done at cell
densities twice that of LB Broth. Cell yields are
typically about 3 to 4 times that of LB Broth in shake flask
cultures depending on the characteristics of the strain.
Plasmid copy numbers are the highest among the AthenaES™
family of media. This medium is an excellent choice
for large-scale plasmid preparations. Biomass yields
will depend on the process employed and the strain.
Formula – Proprietary
Method of Preparation
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Dissolve 35 grams in 1 liter deionized water.
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Dispense as required.
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Autoclave at 121°C for 15 min.
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Media
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Unit Size
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Cat. No.
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500 g
2 pack
6 packa
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0111-1
0111-2
0111-6
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Power
Prime Broth™ is a proprietary medium formulation
developed by AthenaES™ for improved expression of
recombinant proteins in E. coli. This medium is
certified "animal product free" and is a derivative of the
Power Broth™ formula. The medium is a complex, rich
formulation which supplies amino acids, vitamins, and a
carbon source at higher levels than Terrific Broth5.
The medium is buffered at pH 7.2±0.2. For expression
of recombinant proteins, induction should be done at cell
densities three times that of LB Broth. Cell yields
are typically 3 to 4 times that of LB Broth in shake flask
cultures depending on the characteristics of the strain.
The amount of soluble protein accumulated for otherwise
insoluble proteins is increased by growing in Power Broth™.
This medium is suitable as the base for fermentation
cultures. Biomass yields will depend on the process
and the strain employed.
Formula – Proprietary
Method of Preparation
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Dissolve 52 grams in 1 liter deionized water.
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Add 4 ml of glycerol.
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Dispense as required.
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Autoclave at 121°C for 15 min
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Media
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Unit Size
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Cat. No.
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|
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500 g
2 pack
6 packa
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0112-1
0112-2
0112-6
|
a
Six pack sold as six 500 g containers to ensure freshness
during storage, prevent unintentional contamination during
use, and prevent caking of powder.
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Last modified:
05/19/16
|
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