Storage and Stability

• EndoMax Supplement is stored under -20° C at our facility and is shipped on dry ice.

•   If the product is to be used immediately, thaw in a 37° C water bath or overnight at 4° C.

•     If thawed in a water bath, do not leave the product at 37° C for no more than 1 hour.

•    When stored at 4° C, EndoMax Supplement is stable for more than 10 days.

•      If the product is not to be used within 10 days after receipt, we recommend storing it at below -20° C in a freezer that is not self-defrosting.

•    Do not thaw and refreeze more than once. When stored at below -20°C, the product is stable until the expiration date shown on the label.

Preparation of Supplemented Culture Medium

For routine culture of human endothelial cells, add one bottle (25 ml) EndoMax Supplement per 500 ml of an appropriate basal medium.

·        Medium culture 500 ml (in a 500ml bottle)

·        Antibiotics

·        HEPES 6mM

·        Foetal Bovine Serum (5-20%) decomplemented at 56°C for 30 min.

·        The content of 1 vial of EndoMax Supplement GENT-19-EM

Keep the bottle at 4°C until used.

human endothelial cells

human endothelial cells

human endothelial cells

human endothel

human endothelial cells

ial cells

human endothelial cells

Handling:

Good laboratory technique should be employed in the safe handling of this product.

 This requires observing the following practices:

•   Wear appropriate laboratory attire including a lab coat, gloves and safety glasses.

•  Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body which comes into contact with the product.

• Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.

• Handle the product under sterile area.

• This product is intended for research purposes by qualified personnel only. It is not intended for use in humans or in animals. Gentaur is not liable for any damages resulting from the misuse or handling of this product.

human endothelial cells

human endothelial

human endothelial cells

 cells

Components

human endothelial

human

 endothelial cells

dothelial cells

human endothelial cells

human endothelial cells

One bottle of 15 ml.