Diethylstilbestrol ELISA Kit

Brief
Diethylstilbestrol(DES) ELISA Test Kit

Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Diethylstilbestrol(DES) in the feed, urine, liver, meat, shrimp, finish. The coupling antigen is pre-coated on the micro-well stripes. The Diethylstilbestrol in the testing sample competes with the coupling antigen pre-coated on the micro-well stripes for the antibodies against Diethylstilbestrol. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Diethylstilbestrol in the sample. This value is compared to the standard curve and the content of the corresponding Diethylstilbestrol is subsequently obtained.

Specifications
Sensitivity : 0.1ppb
Detection limit
　　Shrimp, finish 0.2ppb
　　Meat, liver 2 ppb
　　Urine 0.6 ppb
　　Feed 20ppb
Recovery rate
　　Urine 70 ±10%
　　Feed 90 ±10%
　　Tissue 85 ±10%
Cross-reactions rate
　　DES 100%
　　Dienestrol 38.5%
　　Hexestrol 8.5%
　　Ethinylestradiol < 0.1%