GENTAUR EUROPE  +32 1658 9045 +32 1650 9045BELGIUM1 tel +32 2 732 5688 fax +32 2 732 4414 [email protected] Av. de l' Armée 68 B-1040 Brussels France tel 01 43 25 01 50 fax 01 43 25 01 60 9, rue Lagrange 75005 Paris Italy tel 02 36 00 65 93 fax +32 16 50 90 45 20135 Milano Germany tel +32 16 58 90 45 fax +32 16 50 90 45 Forckenbeckstraße 6 D-52074 Aachen Japan tel +81 78 386 0860 fax +81 78 306 0296 Minaatojimaminami-manchi Chuo-ku, Kobe 065-0047  |
Diazepam ELISA Kit
Brief
Diazepam ELISA Test Kit
Principle
This test kit is based on the competitive enzyme
immunoassay for the detection of Diazepam in the feed,urine, liver, meat. The
coupling antigen is pre-coated on the micro-well stripes. The Diazepam in the
testing sample competes with the coupling antigen pre-coated on the micro-well
stripes, to interact with the antibodies against Diazepam. After the addition of
the enzyme conjugate, the TMB substrate is added for coloration. The optical
density (OD) value of the testing sample has a negative correlation with the
content of Diazepam in the sample. This value is compared to the standard curve
and the content of the corresponding Diazepam is subsequently obtained.
Specifications
Sensitivity : 0.1ppb
Detection limit
Animal tissue 1ppb
Urine 1ppb
Compound feed 10ppb
Concentrated feed 20ppb
Premixed feed 20ppb
Recovery rate
Urine 80 ±10%
Feed 75 ±10%
Meat,liver(pork) 75 ±10%
Cross-reactions rate
DiaZepatn 100%
Nitrazepam 7.6%
Oxazepam 8.8%
