Custom peptide
polyclonal antisera have become an instrument of paramount importance in
the life science tool-box. However, raising peptide polyclonal
antibodies is a laborious process and for a successful outcome, numerous
pitfalls have to be avoided.
Gentaur offers a
complete set of services, from peptide design to affinity purification.
Whatever your requirements are, we are confident that we will be able to
accommodate you.
Though every project is different, we have a standard custom peptide
polyclonal antiserum package. This package can be extended according to
your specifications and you will also be able to make modifications and
additions during the course of a project.
The
standard package includes:
Peptide Design
Peptide Synthesis,
25 mg
Peptide-Carrier
Conjugation
12 week immunisation
protocol for 1 rabbit according to:
Day 1 Collection
of pre-immune serum
Day 1 Primary immunisation
Day 14 First booster
Day 28 Second booster
Day 42 First serum sample ~10 ml
Day 49 Third booster
Day 63 Second serum sample ~10 ml
Day 70 Fourth booster
Day 84 Production lot ~40 ml
ELISA analysis of
the first serum sample.
Documentation of the
project.
1200 EUR
Additional services are available such as:
Continuation of
project
180 EUR per four-week cycle
Additional ELISA
analyses
120 EUR per analysis
Affinity
Purification of antisera
735 EUR
Supplying of
antiserum in aliquots
60 EUR per serum sample
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