The C494 (MDR) monoclonal antibody detects a gene-specific, internal cellular epitope present only on the MDR1 isoform of P-glycoprotein, a 170 Kd transmembrane glycoprotein. C494 does not cross react with MDR3 P-glycoprotein. P-glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions and is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P-glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P-glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P-glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias/lymphomas.
Master Cat. No. 8720 Cat. No. 8720-01 Clone: C494 Isotype: IgG2a