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A
NTI-CONJUGATED GLYCINEDESCRIPTION
Polyclonal antisera were raised in rabbits after immunization with the conjugates:
Glycine-glutaraldehyde-carriers.
CONTENTS
The antibody is supplied in a unit size of 50
ml in PBS with 50% glycerol.STORAGE AND HANDLING
When this product is stored undiluted at -20
°C or lower, it is stable for at least 2 years. Multiple freezingand thawing is only possible up to 5 times. The antibodies can be prediluted 10x in PBS containing 0.1%
merthiolate or a mixture of PBS/glycerol (1:1, vol./vol.). At 4
°C these solutions are stable for 2 months.SPECIFICITY
Using a conjugate glycine-glutaraldehyde-protein, antibody specificity was determined in an ELISA test by
competition experiments with the following compounds:
Compound Cross-reactivity ratio
(a)Glycine-G-TH 1
b
-Alanine-G-TH 1 : 107GABA-G-TH 1 : 501
Taurine-G-TH 1 : 1,000
Aspartate-G-TH 1 : 20,000
Glutamate-G-TH 1 : > 20,000
Glycine=G=TH (b) 1 : > 100,000
Glycine 1 : > 100,000
(a) : Glycine-G-PL concentration/unconjugated or conjugated aminoacid concentration at half displacement ;
(b) : Non-reduced conjugate ;
G = Glutaraldehyde, TH = Thyroglobulin.
RECOMMENDED DILUTION
The anti-conjugated glycine antibodies can be diluted 1:2,000 to 1:5,000.
REFERENCES
• CAMPISTRON G., BUIJS R.M. and GEFFARD M., Glycine neurons in the brain and spinal cord. Antibody production and immunocytochemical
localization. Brain Res., (1986) 376, 400-405.
• PEYRET D., CAMPISTRON G., GEFFARD M. and ARAN J.M., Glycine immunoreactivity in the brain-stem auditory and vestibular nuclei of the
guinea pig. Acta Otolaryngol. (Stockh), (1987) 104, 71-76.
