Anti-CLAC-P (NC2-1 region) Polyclonal Antibody

 Catalog Number:         25-24306                                 283 Euro / 333 USD

Lot Number:                R5811 

Quantity:                     100 mg in 100 ml (1 mg/ml) in PBS containing 0.02% sodium azide. 

Description:                 Antibodies against senile plaque (SP) amyloid obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with approximately 50/100 kDa polypeptides in Alzheimer's disease (AD) brains. The 9D2 antigen was identified as CLAC (collagen-like Alzheimer amyloid plaque component), and its precursor as CLAC-P/collagen type XXV. Both secreted and membrane-tethered forms of CLAC-P/collagen type XXV specifically bound to fibrillized Abeta, implicating these proteins in beta-amyloidogenesis and neuronal degeneration in AD.

 Host Species:              Rabbit

 Specificity:                   Reacts with residues 171-183 [NHGFLSADQQLIK] of the NC2-1 region of human and mouse CLAC-P. Detects bands of 50, 70 and 100 kDa in SPs of AD brain, but not in diffuse plaques of non-demented aged individuals, diffuse deposits in sub-cortical structures and cerebellum in AD nor in cerebral amyloid angiopathy.

 Application:                 Western blot – Use at 1:500 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

 Format:                       Affinity purified IgG

 Storage:                       Maintain at +2-8°C for 3 months or at -20°C for longer periods.  Stable for 1 year. Avoid repeated freeze-thaw cycles.

 Reference:                   Hashimoto,T. et. al. EMBO J. 21 (7), 1524-1534 (2002).