GENTAUR EUROPE  +32 1658 9045 +32 1650 9045BELGIUM1 tel +32 2 732 5688 fax +32 2 732 4414 [email protected] Av. de l' Armée 68 B-1040 Brussels France tel 01 43 25 01 50 fax 01 43 25 01 60 9, rue Lagrange 75005 Paris Italy tel 02 36 00 65 93 fax +32 16 50 90 45 20135 Milano Germany tel +32 16 58 90 45 fax +32 16 50 90 45 Forckenbeckstraße 6 D-52074 Aachen Japan tel +81 78 386 0860 fax +81 78 306 0296 Minaatojimaminami-manchi Chuo-ku, Kobe 065-0047  |
TACSTM
Annexin V Kits  Annexin V protocolAnnexin V brochure
|
TACS Annexin V Kits allow rapid, specific, and quantitative identification of apoptosis in individual cells. Annexin V is a calcium-dependent phospholipid binding protein. During apoptosis, an early and ubiquitous event is the exposure of phosphatidylserine at the cell surface. Trevigen offers annexin V conjugated to either FITC or to biotin for the detection of cell surface changes during apoptosis. The annexin V conjugates are supplied with an optimized Binding Buffer and propidium iodide. Propidium iodide may be used on unfixed samples to determine the population of cells that have lost membrane integrity, an indication of late apoptosis or necrosis.
| Features: |
|
||
| Applications: |
|
||
| Annexin V FITC Kits | |||
| Components: | Component | Size | Price |
| Annexin V FITC (A) | 100 µl | 323 € | |
| 10X Binding Buffer (A) | 8 ml | 84 € | |
| Propidium Iodide (A) | 1 ml | 84 € | |
| Annexin V FITC (B) | 250 µl | 665 € | |
| 10X Binding Buffer (B) | 20 ml | 103 € | |
| Propidium Iodide (B) | 2.5 ml | 103 € | |
| (A) 4830-01-K Annexin V FITC Kit 100 Samples
347 € (B) 4830-250-K Annexin V FITC Kit 250 Samples 690 € |
|||
| Annexin V Biotin Kits | |||
| Components: | Component | Size | Price |
| 10X Binding Buffer (A) | 8 ml | 84 € | |
| Propidium Iodide (A) | 1 ml | 84 € | |
| 10X Binding Buffer (B) | 20 ml | 103 € | |
| Propidium Iodide (B) | 2.5 ml | 103 € | |
| Annexin V Biotin (A) | 100 µl | 371 € | |
| Annexin V Biotin (B) | 250 µl | 694 € | |
| (A) 4835-01-K Annexin V Biotin Kit 100 Samples
381 € (B) 4835-250-K Annexin V Biotin Kit 250 Samples 708 € |
|||
| Storage: | Stable for at least one year when stored at 4°C. | ||
| References: | 1. Fadock, V.A., D.R. Voelker,
P.A .Campbell, J.J. Cohen, D.L. Bratton, and P.M. Henson. 1992. Exposure
of phosphatidylserine on the surface of apoptotic lymphocytes triggers
specific recognition and removal by macrophages. J Immunol
148:2207-2216. 2. Tait, J.F., and D. Gibson. 1992. Phospholipid binding of Annexin V: Effects of calcium and membrane phosphatidylserine content. Arch Biochemiand Biophys 298(1):187-191. 3. Meers, P., and T. Mealy. 1993. Calcium-dependent Annexin V binding to phospholipids: stoichiometry, specificity, and the role of negative charge. Biochemistry 32:11711-11721. |
||
